RGD Reference Report - Molecular cloning, sequencing, and expression of cDNA for rat liver microsomal aldehyde dehydrogenase. - Rat Genome Database

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Molecular cloning, sequencing, and expression of cDNA for rat liver microsomal aldehyde dehydrogenase.

Authors: Miyauchi, K  Masaki, R  Taketani, S  Yamamoto, A  Akayama, M  Tashiro, Y 
Citation: Miyauchi K, etal., J Biol Chem 1991 Oct 15;266(29):19536-42.
RGD ID: 61536
Pubmed: PMID:1717467   (View Abstract at PubMed)

The cDNA clone for rat liver microsomal aldehyde dehydrogenase (msALDH) was isolated and sequenced. The deduced amino acid sequence consisting of 484 amino acid residues revealed that the carboxyl-terminal region of msALDH has a hydrophobic segment, which is probably important for the insertion of this enzyme into the endoplasmic reticulum membrane. COS-1 cells transfected with the expression vector pcD containing the full-length cDNA showed that the active enzyme was expressed and localized mainly on the cytoplasmic surface of the endoplasmic reticulum membranes. It has been proposed that ALDH isozymes form a superfamily consisting of class 1, 2, and 3 ALDHs (Hempel, J., Harper, K., and Lindahl, R., (1989) Biochemistry 28, 1160-1167). Comparison of the amino acid sequence of rat liver msALDH with those of rat other class ALDHs showed that msALDH was 24.2, 24.0, and 65.5% identical to phenobarbital-inducible ALDH (variant class 1), mitochondrial ALDH (class 2), and tumor-associated ALDH (class 3), respectively. Several amino acid residues common to the other known ALDHs, however, were found to be conserved in msALDH. Based on these results, we proposed to classify msALDH as a new type, class 4 ALDH.



Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Aldh3a2Ratendoplasmic reticulum membrane  IDA  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Aldh3a2Rat3-chloroallyl aldehyde dehydrogenase activity  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Aldh3a2  (aldehyde dehydrogenase 3 family, member A2)


Additional Information