RGD Reference Report - Activation of the rat serine proteinase inhibitor 3 gene by interferon gamma via the interleukin 6-responsive element. - Rat Genome Database

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Activation of the rat serine proteinase inhibitor 3 gene by interferon gamma via the interleukin 6-responsive element.

Authors: Kordula, T  Travis, J 
Citation: Kordula T and Travis J, Biochem J. 1995 Jul 1;309 ( Pt 1):63-7.
RGD ID: 5147424
Pubmed: PMID:7619083   (View Abstract at PubMed)
PMCID: PMC1135800   (View Article at PubMed Central)

Transcription of rat serine proteinase inhibitor 3 (SPI-3) gene is rapidly induced in the liver in response to inflammation. Treatment of rat hepatoma H-35 cells with interferon gamma (INF gamma) results in the immediate induction of this gene, with its 147 bp-long promoter being sufficient for activation. Within this promoter we have identified an IFN gamma-responsive element which maps to the signal transducer and activator of transcription (Stat)3-binding site. Mutation of this element causes a loss of responsiveness to IFN gamma, whereas fusion to a heterologous promoter confers a positive response on IFN gamma. The latter apparently induces the binding of a protein, identified as Stat1, to the described element, which gradually decreases within 24 h. Thus the induction of the SPI-3 gene by IFN gamma correlates with the binding of Stat1 to a specific element which, in turn, binds Stat3 in response to interleukin 6.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Serpina3nRatcellular response to type II interferon  IEP  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Serpina3n  (serpin family A member 3N)


Additional Information