RGD Reference Report - Ordered recruitment of histone acetyltransferases and the TRAP/Mediator complex to thyroid hormone-responsive promoters in vivo. - Rat Genome Database

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Ordered recruitment of histone acetyltransferases and the TRAP/Mediator complex to thyroid hormone-responsive promoters in vivo.

Authors: Sharma, D  Fondell, JD 
Citation: Sharma D and Fondell JD, Proc Natl Acad Sci U S A. 2002 Jun 11;99(12):7934-9. Epub 2002 May 28.
RGD ID: 5134968
Pubmed: PMID:12034878   (View Abstract at PubMed)
PMCID: PMC122998   (View Article at PubMed Central)
DOI: DOI:10.1073/pnas.122004799   (Journal Full-text)

Transcriptional coactivators implicated in gene activation by the thyroid hormone receptor (TR) include members of the p160/steroid receptor coactivator (SRC) family of proteins, p300, and the multisubunit TR-associated protein (TRAP)/Mediator complex. We investigated the temporal recruitment of these cofactors to mammalian thyroid hormone (T3)-responsive promoters in vivo. We show that upon T3 treatment, TR recruits all three types of coactivators to specific promoters in at least two sequential steps: p160/SRC proteins and p300 are recruited first and rapidly induce histone acetylation, followed by the recruitment of the TRAP/Mediator complex. Interestingly, inhibition of histone deacetylase activity with trichostatin A elicited a more rapid promoter recruitment of the TRAP/Mediator complex but not p160/SRC proteins. T3-dependent gene expression assays indicate that all three coactivators are targeted to a promoter before significant activation occurs. These findings thus suggest that histone acetylation may be a prerequisite for TRAP/Mediator recruitment and function at specific T3-responsive mammalian promoters.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Med1Ratcellular response to thyroid hormone stimulus  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Med1  (mediator complex subunit 1)


Additional Information