RGD Reference Report - Mind bomb-2 is an E3 ligase that ubiquitinates the N-methyl-D-aspartate receptor NR2B subunit in a phosphorylation-dependent manner. - Rat Genome Database

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Mind bomb-2 is an E3 ligase that ubiquitinates the N-methyl-D-aspartate receptor NR2B subunit in a phosphorylation-dependent manner.

Authors: Jurd, Rachel  Thornton, Claire  Wang, Jun  Luong, Ken  Phamluong, Khanhky  Kharazia, Viktor  Gibb, Stuart L  Ron, Dorit 
Citation: Jurd R, etal., J Biol Chem. 2008 Jan 4;283(1):301-310. doi: 10.1074/jbc.M705580200. Epub 2007 Oct 25.
RGD ID: 405650227
Pubmed: PMID:17962190   (View Abstract at PubMed)
PMCID: PMC3124443   (View Article at PubMed Central)
DOI: DOI:10.1074/jbc.M705580200   (Journal Full-text)

The N-methyl-D-aspartate receptor (NMDAR) plays a critical role in synaptic plasticity. Post-translational modifications of NMDARs, such as phosphorylation, alter both the activity and trafficking properties of NMDARs. Ubiquitination is increasingly being recognized as another post-translational modification that can alter synaptic protein composition and function. We identified Mind bomb-2 as an E3 ubiquitin ligase that interacts with and ubiquitinates the NR2B subunit of the NMDAR in mammalian cells. The protein-protein interaction and the ubiquitination of the NR2B subunit were found to be enhanced in a Fyn phosphorylation-dependent manner. Immunocytochemical studies reveal that Mind bomb-2 is localized to postsynaptic sites and colocalizes with the NMDAR in apical dendrites of hippocampal neurons. Furthermore, we show that NMDAR activity is down-regulated by Mind bomb-2. These results identify a specific E3 ubiquitin ligase as a novel interactant with the NR2B subunit and suggest a possible mechanism for the regulation of NMDAR function involving both phosphorylation and ubiquitination.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Mib2Ratregulation of postsynaptic neurotransmitter receptor internalization involved_inIDA PMID:17962190SynGO 
Mib2Ratregulation protein catabolic process at postsynapse involved_inIDA PMID:17962190SynGO 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Mib2Ratglutamatergic synapse is_active_inIDA PMID:17962190SynGO 
Mib2Ratpostsynapse is_active_inIDA PMID:17962190SynGO 

Objects Annotated

Genes (Rattus norvegicus)
Mib2  (MIB E3 ubiquitin protein ligase 2)


Additional Information