RGD Reference Report - Glycosylated queuosines in tRNAs optimize translational rate and post-embryonic growth.

Advanced Search (OLGA)

General

Glycosylated queuosines in tRNAs optimize translational rate and post-embryonic growth.
Authors:	Zhao, Xuewei Ma, Ding Ishiguro, Kensuke Saito, Hironori Akichika, Shinichiro Matsuzawa, Ikuya Mito, Mari Irie, Toru Ishibashi, Kota Wakabayashi, Kimi Sakaguchi, Yuriko Yokoyama, Takeshi Mishima, Yuichiro Shirouzu, Mikako Iwasaki, Shintaro Suzuki, Takeo Suzuki, Tsutomu
Citation:	Zhao X, etal., Cell. 2023 Dec 7;186(25):5517-5535.e24. doi: 10.1016/j.cell.2023.10.026. Epub 2023 Nov 21.
RGD ID:	401966881
Pubmed:	PMID:37992713 (View Abstract at PubMed)
DOI:	DOI:10.1016/j.cell.2023.10.026 (Journal Full-text)
Transfer RNA (tRNA) modifications are critical for protein synthesis. Queuosine (Q), a 7-deaza-guanosine derivative, is present in tRNA anticodons. In vertebrate tRNAs for Tyr and Asp, Q is further glycosylated with galactose and mannose to generate galQ and manQ, respectively. However, biogenesis and physiological relevance of Q-glycosylation remain poorly understood. Here, we biochemically identified two RNA glycosylases, QTGAL and QTMAN, and successfully reconstituted Q-glycosylation of tRNAs using nucleotide diphosphate sugars. Ribosome profiling of knockout cells revealed that Q-glycosylation slowed down elongation at cognate codons, UAC and GAC (GAU), respectively. We also found that galactosylation of Q suppresses stop codon readthrough. Moreover, protein aggregates increased in cells lacking Q-glycosylation, indicating that Q-glycosylation contributes to proteostasis. Cryo-EM of human ribosome-tRNA complex revealed the molecular basis of codon recognition regulated by Q-glycosylations. Furthermore, zebrafish qtgal and qtman knockout lines displayed shortened body length, implying that Q-glycosylation is required for post-embryonic growth in vertebrates.

Annotation Click to see Annotation Detail View

Gene Ontology Annotations Click to see Annotation Detail View

Biological Process

Only show annotations with direct experimental evidence (0 objects hidden)

Object Symbol	Species	Term	Qualifier	Evidence	With	Notes	Source	Original Reference(s)
B3gntl1	Rat	tRNA modification	involved_in	IDA		PMID:37992713	UniProt
Gtdc1	Rat	tRNA modification	involved_in	IDA		PMID:37992713	UniProt

Molecular Function

Only show annotations with direct experimental evidence (0 objects hidden)

Object Symbol	Species	Term	Qualifier	Evidence	With	Notes	Source	Original Reference(s)
Gtdc1	Rat	tRNA-queuosine(34) beta-mannosyltransferase activity	enables	IDA		PMID:37992713	UniProt
B3gntl1	Rat	tRNA-queuosine(34) galactosyltransferase activity	enables	IDA		PMID:37992713	UniProt

Objects Annotated

Genes (Rattus norvegicus)

B3gntl1 (UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase-like 1)

Gtdc1 (glycosyltransferase-like domain containing 1)

Additional Information

Gene Annotator (Functional Annotation) unavailable	Gene Annotator (Annotation Distribution) unavailable	Variant Visualizer (Genomic Variants) unavailble Variant Visualizer (Genomic Variants) unavailable
Gene Annotator (Functional Annotation)	Gene Annotator (Annotation Distribution)	Variant Visualizer (Genomic Variants)

InterViewer (Protein-Protein Interactions) unavailable	Gviewer (Genome Viewer) unavailable	Variant Visualizer (Damaging Variants) unavailble Variant Visualizer (Damaging Variants) unavailable
InterViewer (Protein-Protein Interactions)	GViewer (Genome Viewer)	Variant Visualizer (Damaging Variants)

Gene Annotator (Annotation Comparison) unavailable	OLGA (Gene List Generator) unavailable
Gene Annotator (Annotation Comparison)	OLGA (Gene List Generator)	Excel (Download)

MOET (Multi-Ontology Enrichement) unavailable	GOLF (Gene-Ortholog Location Finder) unavailable
MOET (Multi-Ontology Enrichement)	GOLF (Gene-Ortholog Location Finder)

Create Name:

Description:

Send us a Message

Glycosylated queuosines in tRNAs optimize translational rate and post-embryonic growth.