RGD Reference Report - Phospholipid transfer activity of microsomal triacylglycerol transfer protein is sufficient for the assembly and secretion of apolipoprotein B lipoproteins. - Rat Genome Database

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Phospholipid transfer activity of microsomal triacylglycerol transfer protein is sufficient for the assembly and secretion of apolipoprotein B lipoproteins.

Authors: Rava, Paul  Ojakian, George K  Shelness, Gregory S  Hussain, M Mahmood 
Citation: Rava P, etal., J Biol Chem. 2006 Apr 21;281(16):11019-27. doi: 10.1074/jbc.M512823200. Epub 2006 Feb 13.
RGD ID: 39458033
Pubmed: PMID:16478722   (View Abstract at PubMed)
DOI: DOI:10.1074/jbc.M512823200   (Journal Full-text)

Human microsomal triacylglycerol transfer protein (hMTP) is essential for apolipoprotein B (apoB)-lipoprotein assembly and secretion and is known to transfer triacylglycerols, cholesterol esters, and phospholipids. To understand the relative importance of each lipid transfer activity, we compared the ability of hMTP and its Drosophila ortholog (dMTP) to assemble apoB lipoproteins and to transfer various lipids. apoB48 secretion was induced when co-expressed with either hMTP or dMTP in COS cells, and oleic acid supplementation further augmented secretion without altering particle density. C-terminal epitope-tagged dMTP (dMTP-FLAG) facilitated the secretion of apoB polypeptides in the range of apoB48 to apoB72 but was approximately 50% as efficient as hMTP-FLAG. Comparison of lipid transfer activities revealed that although phospholipid transfer was similar in both orthologs, dMTP was unable to transfer neutral lipids. We conclude that the phospholipid transfer activity of MTP is sufficient for the assembly and secretion of primordial apoB lipoproteins and may represent its earliest function evolved for the mobilization of lipid in invertebrates. Identification of MTP inhibitors, which selectively affect transfer of a specific lipid class, may have therapeutic potential.



Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
MttpRatphosphatidylcholine transfer activity enablesIMP PMID:16478722UniProt 
MttpRattriglyceride transfer activity enablesIDA PMID:16478722UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Mttp  (microsomal triglyceride transfer protein)


Additional Information