RGD Reference Report - HBsAg stimulates NKG2D receptor expression on natural killer cells and inhibits hepatitis C virus replication. - Rat Genome Database

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HBsAg stimulates NKG2D receptor expression on natural killer cells and inhibits hepatitis C virus replication.

Authors: Wang, Xiao-Xiao  Pan, Xiao-Ben  Han, Jin-Chao  Cong, Xu  Jin, Qian  Kong, Xiang-Sha  Wei, Lai  Feng, Bo 
Citation: Wang XX, etal., Hepatobiliary Pancreat Dis Int. 2018 Jun;17(3):233-240. doi: 10.1016/j.hbpd.2018.03.010. Epub 2018 Mar 26.
RGD ID: 39128177
Pubmed: PMID:29625837   (View Abstract at PubMed)
DOI: DOI:10.1016/j.hbpd.2018.03.010   (Journal Full-text)


BACKGROUND: Higher hepatitis B surface antigen (HBsAg) facilitates hepatitis C virus (HCV) clearance in patients with hepatitis B virus (HBV)/HCV co-infection. We investigated the effect of exogenous HBsAg on the inhibition of HCV replication mediated by natural killer (NK) cells.
METHODS: After isolated from peripheral blood of 42 chronic hepatitis B (CHB) patients and 16 healthy individuals, NK cells were co-cultured with HCV-infected Huh7 cells, respectively, with or without HBsAg. Three days later, the co-cultured supernatants were collected and HCV RNA levels were measured by real-time quantitative PCR. NKG2D, NKp46 and NKG2A expression levels were measured by flow cytometry. NKG2D on NK cells from CHB responsive subgroup was blocked and HCV RNA levels were examined again.
RESULTS: HCV RNA levels in the co-cultured system were significantly reduced by NK cells isolated from healthy donors (P < 0.01) but not from CHB patients. However, HCV RNA levels in CHB cultures were significantly decreased following HBsAg addition (P < 0.05), whereas no such effect was seen in control cultures. No significant difference was observed in basic NKG2D expression between the CHB patients and healthy donors. On NK cells from CHB patients, the expression of NKG2D was increased significantly by HBsAg stimulation (P < 0.01), and higher than that from healthy controls (P < 0.05). HCV RNA levels were increased significantly after the blockage of NKG2D on NK cells from responsive CHB patients in the co-cultured system (P < 0.05).
CONCLUSION: Exogenous HBsAg stimulated NKG2D expression on NK cells from CHB patients which inhibit HCV replication, suggesting that HBsAg may facilitate the clearance of HCV in patients with HBV/HCV co-infection.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
NCR1HumanChronic Hepatitis B  IEP protein:decreased expression:Peripheral blood mononuclear cell and natural killer cell (human)RGD 
Ncr1RatChronic Hepatitis B  ISONCR1 (Homo sapiens)protein:decreased expression:Peripheral blood mononuclear cell and natural killer cell (human)RGD 
Ncr1MouseChronic Hepatitis B  ISONCR1 (Homo sapiens)protein:decreased expression:Peripheral blood mononuclear cell and natural killer cell (human)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Ncr1  (natural cytotoxicity triggering receptor 1)

Genes (Mus musculus)
Ncr1  (natural cytotoxicity triggering receptor 1)

Genes (Homo sapiens)
NCR1  (natural cytotoxicity triggering receptor 1)


Additional Information