RGD Reference Report - Regulation of neurite outgrowth by interactions between the scaffolding protein, JNK-associated leucine zipper protein, and neuronal growth-associated protein superior cervical ganglia clone 10. - Rat Genome Database

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Regulation of neurite outgrowth by interactions between the scaffolding protein, JNK-associated leucine zipper protein, and neuronal growth-associated protein superior cervical ganglia clone 10.

Authors: Xu, Hua  Dhanasekaran, Danny N  Lee, Clement M  Reddy, E Premkumar 
Citation: Xu H, etal., J Biol Chem. 2010 Feb 5;285(6):3548-53. doi: 10.1074/jbc.M109.064113. Epub 2009 Dec 3.
RGD ID: 30296662
Pubmed: PMID:19959466   (View Abstract at PubMed)
PMCID: PMC2823494   (View Article at PubMed Central)
DOI: DOI:10.1074/jbc.M109.064113   (Journal Full-text)

JLP (JNK-associated leucine zipper protein) is a novel scaffolding protein involved in JNK signaling. Although it is known that JLP is highly expressed in brain, the biological function of JLP in neuronal systems remains unknown. Here, we report a novel interaction between JLP and SCG10 (superior cervical ganglia clone 10), which is a microtubule-destabilizing factor that is essential for neurite outgrowth. Inhibition of endogenous JLP expression using small interference RNA methodology strongly enhanced nerve growth factor (NGF)-induced neurite outgrowth in PC12 cells. Our results show that JLP negatively regulates NGF-induced neurite outgrowth by decreasing the level of phosphorylated SCG10. Furthermore, inhibition of JNK phosphorylation by a small molecule inhibitor, SP600125, resulted in inhibition of SCG10 phosphorylation and inhibition of neurite growth. Taken together, our results suggest that JLP negatively regulates NGF-induced neurite outgrowth through a sequestering mechanism that results in an attenuation of NGF-induced SCG10 phosphorylation.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Spag9Ratnegative regulation of dendrite extension  IMP  RGD 
Spag9Ratnegative regulation of neuron differentiation  IMP  RGD 
Spag9Ratnegative regulation of protein phosphorylation  IMP  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Spag9  (sperm associated antigen 9)


Additional Information