RGD Reference Report - Genomic response of hypoxic Muller cells involves the very low density lipoprotein receptor as part of an angiogenic network. - Rat Genome Database

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Genomic response of hypoxic Muller cells involves the very low density lipoprotein receptor as part of an angiogenic network.

Authors: Loewen, N  Chen, J  Dudley, VJ  Sarthy, VP  Mathura JR, JR 
Citation: Loewen N, etal., Exp Eye Res. 2009 May;88(5):928-37. Epub 2009 Jan 8.
RGD ID: 2324641
Pubmed: PMID:19233325   (View Abstract at PubMed)
DOI: DOI:10.1016/j.exer.2008.11.037   (Journal Full-text)

Muller cells have recently been found to produce select angiogenic substances. In choosing a more comprehensive approach, we wanted to study the genomic response of Muller cells to hypoxia to identify novel angiogenic genes. An established Muller cell line (rMC-1) was exposed to standard or hypoxic conditions. We analyzed gene expression with three independent microarrays and determined differential expression levels compared to normoxia. Selected genes were confirmed by real-time PCR (RTPCR). Subcellular localization of proteins was examined by immunocytochemistry. A network-based pathway analysis was performed to investigate how those genes may contribute to angiogenesis. We found 19,004 of 28,000 known rat genes expressed in Muller cells. 211 genes were upregulated by hypoxia 1.5 to 14.9-fold (p<0.001, FDR



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
VldlrRatcellular response to hypoxia  IDA  RGD 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
VldlrRatcell surface  IDA  RGD 
VldlrRatperinuclear region of cytoplasm  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Vldlr  (very low density lipoprotein receptor)


Additional Information