RGD Reference Report - A novel testis protein, RSB-66, interacting with INCA1 (inhibitor of Cdk interacting with cyclin A1). - Rat Genome Database

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A novel testis protein, RSB-66, interacting with INCA1 (inhibitor of Cdk interacting with cyclin A1).

Authors: Chen, X  Hu, T  Liang, G  Yang, M  Zong, S  Miao, S  Koide, SS  Wang, L 
Citation: Chen X, etal., Biochem Cell Biol. 2008 Aug;86(4):345-51.
RGD ID: 2316298
Pubmed: PMID:18756329   (View Abstract at PubMed)
DOI: DOI:10.1139/o08-072   (Journal Full-text)

rsb-66 is a novel gene from a suppression subtracted hybridization (SSH) library of round spermatid-specific cDNAs against those of primary spermatocytes. It was found to be specifically expressed in round spermatids. To explore the function of RSB-66, a yeast two-hybrid system was used to screen for potential interacting partners in a human testis cDNA library. HSD45, also known as INCA1 (inhibitor of Cdk interacting with cyclin A1), was identified as one of the positive clones. The interaction between RSB-66 and INCA1 was demonstrated to occur by GST pull down and coimmunoprecipitation. Using immunofluorescence, RSB-66 was found to be specifically expressed in round spermatids, mainly in the cytoplasm. When being transfected into HeLa cells, RSB-66 and INCA1 were found to be co-localized principally in the cytoplasm. The alpha helix in the RSB-66 C terminal and two amino acid residues (tyr117 and his119) appear to be crucial for its function.



Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Spaca9Ratcytoplasm located_inIDA PMID:18756329UniProt 
Spaca9Ratnucleus located_inIDA PMID:18756329UniProt 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Spaca9Ratprotein binding enablesIPIUniProtKB:Q0VD86PMID:18756329UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Spaca9  (sperm acrosome associated 9)

Objects referenced in this article
Gene Inca1 inhibitor of CDK, cyclin A1 interacting protein 1 Rattus norvegicus

Additional Information