RGD Reference Report - Characterization of a novel gene, STAG1/PMEPA1, upregulated in renal cell carcinoma and other solid tumors. - Rat Genome Database

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Characterization of a novel gene, STAG1/PMEPA1, upregulated in renal cell carcinoma and other solid tumors.

Authors: Rae, FK  Hooper, JD  Nicol, DL  Clements, JA 
Citation: Rae FK, etal., Mol Carcinog. 2001 Sep;32(1):44-53.
RGD ID: 2315108
Pubmed: PMID:11568975   (View Abstract at PubMed)

Using differential display-polymerase chain reaction, we identified a novel gene sequence, designated solid tumor-associated gene 1 (STAG1), that is upregulated in renal cell carcinoma (RCC). The full-length cDNA (4839 bp) encompassed the recently reported androgen-regulated prostatic cDNA PMEPA1, and so we refer to this gene as STAG1/PMEPA1. Two STAG1/PMEPA1 mRNA transcripts of approximately 2.7 and 5 kb, with identical coding regions but variant 3' untranslated regions, were predominantly expressed in normal prostate tissue and at lower levels in the ovary. The expression of this gene was upregulated in 87% of RCC samples and also was upregulated in stomach and rectal adenocarcinomas. In contrast, STAG1/PMEPA1 expression was barely detectable in leukemia and lymphoma samples. Analysis of expressed sequence tag databases showed that STAG1/PMEPA1 also was expressed in pancreatic, endometrial, and prostatic adenocarcinomas. The STAG1/PMEPA1 cDNA encodes a 287-amino-acid protein containing a putative transmembrane domain and motifs that suggest that it may bind src homology 3- and tryptophan tryptophan domain-containing proteins. This protein shows 67% identity to the protein encoded by the chromosome 18 open reading frame 1 gene. Translation of STAG1/PMEPA1 mRNA in vitro showed two products of 36 and 39 kDa, respectively, suggesting that translation may initiate at more than one site. Comparison to genomic clones showed that STAG1/PMEPA1 was located on chromosome 20q13 between microsatellite markers D20S183 and D20S173 and spanned four exons and three introns. The upregulation of this gene in several solid tumors indicated that it may play an important role in tumorigenesis.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
PMEPA1Humangastric adenocarcinoma  IEP mRNA:increased expression:stomachRGD 
Pmepa1Ratgastric adenocarcinoma  ISOPMEPA1 (Homo sapiens)mRNA:increased expression:stomachRGD 
Pmepa1Mousegastric adenocarcinoma  ISOPMEPA1 (Homo sapiens)mRNA:increased expression:stomachRGD 
PMEPA1Humanrectal benign neoplasm  IEP mRNA:increased expression:rectumRGD 
Pmepa1Ratrectal benign neoplasm  ISOPMEPA1 (Homo sapiens)mRNA:increased expression:rectumRGD 
Pmepa1Mouserectal benign neoplasm  ISOPMEPA1 (Homo sapiens)mRNA:increased expression:rectumRGD 
PMEPA1Humanrenal cell carcinoma  IEP mRNA:increased expression:kidneyRGD 
Pmepa1Ratrenal cell carcinoma  ISOPMEPA1 (Homo sapiens)mRNA:increased expression:kidneyRGD 
Pmepa1Mouserenal cell carcinoma  ISOPMEPA1 (Homo sapiens)mRNA:increased expression:kidneyRGD 

Objects Annotated

Genes (Rattus norvegicus)
Pmepa1  (prostate transmembrane protein, androgen induced 1)

Genes (Mus musculus)
Pmepa1  (prostate transmembrane protein, androgen induced 1)

Genes (Homo sapiens)
PMEPA1  (prostate transmembrane protein, androgen induced 1)


Additional Information