RGD Reference Report - Identification of mammalian Mediator subunits with similarities to yeast Mediator subunits Srb5, Srb6, Med11, and Rox3. - Rat Genome Database

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Identification of mammalian Mediator subunits with similarities to yeast Mediator subunits Srb5, Srb6, Med11, and Rox3.

Authors: Sato, S  Tomomori-Sato, C  Banks, CA  Sorokina, I  Parmely, TJ  Kong, SE  Jin, J  Cai, Y  Lane, WS  Brower, CS  Conaway, RC  Conaway, JW 
Citation: Sato S, etal., J Biol Chem. 2003 Apr 25;278(17):15123-7. Epub 2003 Feb 12.
RGD ID: 2304263
Pubmed: PMID:12584197   (View Abstract at PubMed)
DOI: DOI:10.1074/jbc.C300054200   (Journal Full-text)

The Mediator is a multiprotein coactivator required for activation of RNA polymerase II transcription by DNA binding transactivators. We recently identified a mammalian homologue of yeast Mediator subunit Med8 and partially purified a Med8-containing Mediator complex from rat liver nuclei (Brower, C. S., Sato, S., Tomomori-Sato, C., Kamura, T., Pause, A., Stearman, R., Klausner, R. D., Malik, S., Lane, W. S., Sorokina, I., Roeder, R. G., Conaway, J. W., and Conaway, R. C. (2002) Proc. Natl. Acad. Sci. U. S. A. 99, 10353-10358). Analysis of proteins present in the most highly purified Med8-containing fractions by tandem mass spectrometry led to the identification of many known mammalian Mediator subunits, as well as four potential Mediator subunits exhibiting sequence similarity to yeast Mediator subunits Srb5, Srb6, Med11, and Rox3. Here we present direct biochemical evidence that these four proteins are bona fide mammalian Mediator subunits. In addition, we identify direct pairwise binding partners of these proteins among the known mammalian Mediator subunits. Taken together, our findings identify a collection of novel mammalian Mediator subunits and shed new light on the underlying architecture of the mammalian Mediator complex.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Med11Ratprotein ubiquitination  IDA  RGD 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Med11Ratubiquitin ligase complex  IDA  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Med11Ratubiquitin protein ligase activity contributes_toIDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Med11  (mediator complex subunit 11)

Objects referenced in this article
Gene Med31 mediator complex subunit 31 Rattus norvegicus

Additional Information