RGD Reference Report - Control of phosphofructokinase from rat skeletal muscle. Effects of fructose diphosphate, AMP, ATP, and citrate. - Rat Genome Database

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Control of phosphofructokinase from rat skeletal muscle. Effects of fructose diphosphate, AMP, ATP, and citrate.

Authors: Tornheim, K  Lowenstein, JM 
Citation: Tornheim K and Lowenstein JM, J Biol Chem. 1976 Dec 10;251(23):7322-8.
RGD ID: 2302675
Pubmed: PMID:12161   (View Abstract at PubMed)

Under conditions used previously for demonstrating glycolytic oscillations in muscle extracts (pH 6.65, 0.1 to 0.5 mM ATP), phosphofructokinase from rat skeletal muscle is strongly activated by micromolar concentrations of fructose diphosphate. The activation is dependent on the presence of AMP. Activation by fructose diphosphate and AMP, and inhibition by ATP, is primarily due to large changes in the apparent affinity of the enzyme for the substrate fructose 6-phosphate. These control properties can account for the generation of glycolytic oscillations. The enzyme was also studied under conditions approximating the metabolite contents of skeletal muscle in vivo (pH 7.0, 10mM ATP, 0.1 mM fructose 6-phosphate). Under these more inhibitory conditions, phosphofructokinase is strongly activated by low concentrations of fructose diphosphate, with half-maximal activation at about 10 muM. Citrate is a potent inhibitor at physiological concentrations, whereas AMP is a strong activator. Both AMP and citrate affect the maximum velocity and have little effect on affinity of the enzyme for fructose diphosphate.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
PfkmRatfructose 1,6-bisphosphate metabolic process  IDA  RGD 
PfkmRatfructose 6-phosphate metabolic process  IDA  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
PfkmRat6-phosphofructokinase activity  IDA  RGD 
PfkmRatAMP binding  IDA  RGD 
PfkmRatATP binding  IDA  RGD 
PfkmRatfructose-6-phosphate binding  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Pfkm  (phosphofructokinase, muscle)


Additional Information