RGD Reference Report - NF-kappaB is activated in the rat testis following exposure to mono-(2-ethylhexyl) phthalate. - Rat Genome Database

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NF-kappaB is activated in the rat testis following exposure to mono-(2-ethylhexyl) phthalate.

Authors: Rasoulpour, RJ  Boekelheide, K 
Citation: Rasoulpour RJ and Boekelheide K, Biol Reprod. 2005 Feb;72(2):479-86. Epub 2004 Oct 20.
RGD ID: 2300280
Pubmed: PMID:15496515   (View Abstract at PubMed)
DOI: DOI:10.1095/biolreprod.104.034363   (Journal Full-text)

The process of spermatogenesis requires a delicate balance of proapoptotic and antiapoptotic signaling to maintain optimal sperm output. A major transcription factor known to regulate numerous apoptosis-related genes is NF-kappaB. Here we show that mono-(2-ethylhexyl) phthalate (MEHP, 1 g/kg) induces translocation of NF-kappaB subunits (p65, p50, and c-Rel) to germ cell nuclei in young rats (Postnatal Day 28) as early as 1 h after exposure. Immunohistochemistry of rat testes exposed to MEHP showed increased p50 and c-Rel presence in spermatocytes and spermatogonia. In addition, there was increased p65 nuclear positivity in Sertoli cells and germ cells after MEHP, while Rel-B localization was unchanged. These alterations correlated with increased nuclear NF-kappaB-binding activity after MEHP exposure, as shown by electrophoretic mobility shift assays of whole-testis nuclear protein extracts. The increased activity of this transcription factor was associated with a transient protection of the seminiferous epithelium manifested as a decreased number of germ cell apoptotic nuclei measured by TUNEL assay 6 h after MEHP exposure. These results suggest that NF-kappaB is involved in the testicular response to MEHP-induced injury.



Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
RelRatsequence-specific DNA binding  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Rel  (REL proto-oncogene, NF-kB subunit)


Additional Information