RGD Reference Report - Circulating microRNA profiles in plasma: identification of miR-224 as a novel diagnostic biomarker in hepatocellular carcinoma independent of hepatic function. - Rat Genome Database

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Circulating microRNA profiles in plasma: identification of miR-224 as a novel diagnostic biomarker in hepatocellular carcinoma independent of hepatic function.

Authors: Okajima, Wataru  Komatsu, Shuhei  Ichikawa, Daisuke  Miyamae, Mahito  Kawaguchi, Tsutomu  Hirajima, Shoji  Ohashi, Takuma  Imamura, Taisuke  Kiuchi, Jun  Arita, Tomohiro  Konishi, Hirotaka  Shiozaki, Atsushi  Moriumura, Ryo  Ikoma, Hisashi  Okamoto, Kazuma  Taniguchi, Hiroki  Itoh, Yoshito  Otsuji, Eigo 
Citation: Okajima W, etal., Oncotarget. 2016 Aug 16;7(33):53820-53836. doi: 10.18632/oncotarget.10781.
RGD ID: 18182926
Pubmed: PMID:27462777   (View Abstract at PubMed)
PMCID: PMC5288224   (View Article at PubMed Central)
DOI: DOI:10.18632/oncotarget.10781   (Journal Full-text)


AIMS: This study was designed to identify novel microRNAs (miRNAs) in plasma for detecting and monitoring hepatocellular carcinoma (HCC), independent of hepatic function and background liver diseases with different etiologies.
RESULTS: (1) Four oncogenic miRNAs (miR-151, 155, 191 and 224) with high expression in HCC tissues were selected as candidates. (2) Quantitative RT-PCR using plasma samples from 107 HCC patients and 75 healthy volunteers revealed a significantly higher level of plasma miR-224 in HCC patients than in healthy volunteers according to a small-scale analysis (P < 0.0001), two independent large-scale cohort analysis (P < 0.0001, AUC 0.908). (3) miR-224 expression was significantly higher in HCC tissues and HCC cell lines than in normal hepatic tissues and fibroblasts, respectively. (P = 0.0011, 0.0150) (4) Plasma miR-224 reflected tumor dynamics; preoperative plasma levels of miR-224 were significantly reduced in postoperative samples (P = 0.0058), and plasma miR-224 levels were significantly correlated with paired miR-224 levels in HCC tissues (P = 0.0005). (5) Furthermore, plasma miR-224 levels significantly discriminated HCC patients from patients with chronic liver disease (P = 0.0008). A high plasma miR-224 level was significantly correlated with larger tumor size (P = 0.0005) and recurrences (P = 0.0027). The plasma miR-224 level could accurately detect small tumors less than 18 mm preoperatively.
METHODS: We performed a systematic review of the NCBI database and selected candidate miRNAs reported as highly expressed in HCC tissue.
CONCLUSIONS: Plasma miR-224 may be a sensitive biomarker for screening HCC and monitoring tumor dynamics.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
MIR224Humanhepatocellular carcinoma severityIEP miRNA:increased expression:plasma (human)RGD 
Mir224Mousehepatocellular carcinoma severityIEP miRNA:increased expression:plasma (human)RGD 
Mir224Rathepatocellular carcinoma severityIEP miRNA:increased expression:plasma (human)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Mir224  (microRNA 224)

Genes (Mus musculus)
Mir224  (microRNA 224)

Genes (Homo sapiens)
MIR224  (microRNA 224)


Additional Information