RGD Reference Report - Angiotensin II-dependent increased expression of Na+-glucose cotransporter in hypertension. - Rat Genome Database

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Angiotensin II-dependent increased expression of Na+-glucose cotransporter in hypertension.

Authors: Bautista, R  Manning, R  Martinez, F  Avila-Casado Mdel, C  Soto, V  Medina, A  Escalante, B 
Citation: Bautista R, etal., Am J Physiol Renal Physiol. 2004 Jan;286(1):F127-33. Epub 2003 Sep 23.
RGD ID: 1599050
Pubmed: PMID:14506074   (View Abstract at PubMed)
DOI: DOI:10.1152/ajprenal.00113.2003   (Journal Full-text)

Glucose uptake is increased in hypertension. Thus we investigated Na+-glucose cotransporter (SGLT2) activity and expression in proximal tubules from renovascular hypertensive rats. Sham-operated rats, aortic coarctation rats, and aortic coarctation rats treated with either ramipril (2.5 mg.kg-1.day-1 for 21 days) or losartan (10 mg.kg-1.day-1 for 21 days) were used. Na+-dependent glucose uptake was measured in brush-border membrane vesicles (BBMV). Vmax in BBMV from hypertensive rats was greater compared with those from normotensive rats (3 +/- 0.2 vs. 1.5 +/- 0.1 nmol.mg protein-1.min-1) without a change in Km. Renal immunostaining was greater, and Western blot analysis and RT-PCR showed a higher expression of SGLT2 in hypertensive rats than in normotensive rats (1,029 +/- 71 vs. 5,003 +/- 292, 199 +/- 15 vs. 95 +/- 10, and 1.4 +/- 0.2 vs. 0.3 +/- 0.1 arbitrary units, respectively). In rats treated with either ramipril or losartan, Vmax decreased to 2.1 +/- 0.3 and 1.8 +/- 0.4 nmol.mg protein-1.min-1, respectively, as well as did the intensity of immunostaining and levels of protein and mRNA. We suggest that in renovascular hypertension, angiotensin II induced SGLT2 via the AT1 receptor, which was evidenced at both the functional and expression levels, probably contributing to increased absorption of Na+ and thereby to the development or maintenance of hypertension.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
SLC5A2Humanhypertension  ISOSlc5a2 (Rattus norvegicus) RGD 
Slc5a2Rathypertension  IDA  RGD 
Slc5a2Mousehypertension  ISOSlc5a2 (Rattus norvegicus) RGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Slc5a2RatD-glucose transmembrane transport  IDA  RGD 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Slc5a2Ratbrush border membrane  IDA  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Slc5a2RatD-glucose:sodium symporter activity  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Slc5a2  (solute carrier family 5 member 2)

Genes (Mus musculus)
Slc5a2  (solute carrier family 5 (sodium/glucose cotransporter), member 2)

Genes (Homo sapiens)
SLC5A2  (solute carrier family 5 member 2)


Additional Information