RGD Reference Report - Eukaryotic initiation factor 2B: identification of multiple phosphorylation sites in the epsilon-subunit and their functions in vivo. - Rat Genome Database

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Eukaryotic initiation factor 2B: identification of multiple phosphorylation sites in the epsilon-subunit and their functions in vivo.

Authors: Wang, X  Paulin, FE  Campbell, LE  Gomez, E  O'Brien, K  Morrice, N  Proud, CG 
Citation: Wang X, etal., EMBO J. 2001 Aug 15;20(16):4349-59.
RGD ID: 1581085
Pubmed: PMID:11500362   (View Abstract at PubMed)
PMCID: PMC125262   (View Article at PubMed Central)
DOI: DOI:10.1093/emboj/20.16.4349   (Journal Full-text)

Eukaryotic initiation factor (eIF) 2B is a heteromeric guanine nucleotide exchange factor that plays an important role in regulating mRNA translation. Here we identify multiple phosphorylation sites in the largest, catalytic, subunit (epsilon) of mammalian eIF2B. These sites are phosphorylated by four different protein kinases. Two conserved sites (Ser712/713) are phosphorylated by casein kinase 2. They lie at the extreme C-terminus and are required for the interaction of eIF2Bepsilon with its substrate, eIF2, in vivo and for eIF2B activity in vitro. Glycogen synthase kinase 3 (GSK3) is responsible for phosphorylating Ser535. This regulatory phosphorylation event requires both the fourth site (Ser539) and a distal region, which acts to recruit GSK3 to eIF2Bepsilon in vivo. The fifth site, which lies outside the catalytic domain of eIF2Bepsilon, can be phosphorylated by casein kinase 1. All five sites are phosphorylated in the eIF2B complex in vivo.



Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Eif2b5Ratguanyl-nucleotide exchange factor activity  IMP  RGD 
Eif2b5Ratprotein binding enablesIPIUniProtKB:P05198PMID:11500362UniProt 
Eif2b5Rattranslation initiation factor binding  IMP  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Eif2b5  (eukaryotic translation initiation factor 2B subunit epsilon)


Additional Information