RGD Reference Report - Isolation and characterization of brain Y-box protein: developmentally regulated expression, polyribosomal association and dendritic localization. - Rat Genome Database

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Isolation and characterization of brain Y-box protein: developmentally regulated expression, polyribosomal association and dendritic localization.

Authors: Funakoshi, T  Kobayashi, S  Ohashi, S  Sato, TA  Anzai, K 
Citation: Funakoshi T, etal., Brain Res Mol Brain Res. 2003 Oct 21;118(1-2):1-9.
RGD ID: 1580632
Pubmed: PMID:14559349   (View Abstract at PubMed)

Y-box proteins are DNA- and RNA-binding proteins and control specific gene expression at both transcriptional and translational levels. Particularly in germ cells, it has been reported that Y-box proteins bind to paternal or maternal mRNAs to form mRNPs, mask them from translation and control cell maturation. In this study, we cloned cDNA for a Y-box protein from rat brain. A deduced amino acid sequence of the protein was very similar to that of several other Y-box proteins, and we termed the protein rBYB1 (rat brain Y-box protein 1). rBYB1 was found to be considerably expressed in the cytoplasm of pre- and early postnatal brains, and then decreased to adult levels with brain development. Further, we found rBYB1 to be distributed in both polyribosomal and nonpolyribosomal (mRNP) fractions on a sucrose density gradient, and to be associated with polyribosomes via RNA in the higher-density fractions. Moreover, rBYB1 was localized in dendrites of the primary hippocampal neurons. We compared these sucrose gradient and intracellular rBYB1 localization results with those for fragile X mental retardation protein (FMRP), which is known to be an mRNA-binding and polyribosome-associating translational regulator distributed in neuronal dendrites. Our results suggest that in the brain of prenatal and newborn animals, rBYB1 may function in storage and/or translational regulation of mRNAs involved in the rapid progress of the postnatal brain, and in mature neurons, it may also participate in the control of protein synthesis in dendrites.



Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Ybx1Ratdendrite  IDA  RGD 
Ybx1Ratneuronal cell body  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Ybx1  (Y box binding protein 1)


Additional Information