RGD Reference Report - Prognostic significance of Cytokeratin 20-positive lymph node vascular endothelial growth factor A mRNA and chromodomain helicase DNA binding protein 4 in pN0 colorectal cancer patients. - Rat Genome Database

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Prognostic significance of Cytokeratin 20-positive lymph node vascular endothelial growth factor A mRNA and chromodomain helicase DNA binding protein 4 in pN0 colorectal cancer patients.

Authors: Wong, Sze Chuen Cesar  Cheung, Moon Tong  Luk, Lewis Lai Yin  Lee, Vivian Ha Man  Chan, Pak Tat  Tsang, Hin Fung Andy  Wong, Evelyn Yin Kwan  Xue, Vivian Weiwen  Chan, Amanda Kit Ching  Chan, John Kwok Cheung 
Citation: Wong SCC, etal., Oncotarget. 2017 Dec 19;9(6):6737-6751. doi: 10.18632/oncotarget.23424. eCollection 2018 Jan 23.
RGD ID: 153323304
Pubmed: PMID:29467924   (View Abstract at PubMed)
PMCID: PMC5805510   (View Article at PubMed Central)
DOI: DOI:10.18632/oncotarget.23424   (Journal Full-text)


BACKGROUND: Cytokeratin 20-positive cells in lymph nodes from pN0 colorectal cancer (CRC) patients were detected previously by us. The aims of this study were to investigate which tumor metastasis-related genes were involved and their potential clinical significance.
RESULTS: Fourteen of 84 (17%) genes were differentially expressed by at least 2-fold. Among them, 10 genes were up-regulated whereas 4 genes were down-regulated. Those differential expressed genes were validated in the second cohort of specimens. Follow-up analysis for 60 months showed that patients with lymph node vascular endothelial growth factor A (VEGF-A) mRNA and chromodomain helicase DNA binding protein 4 (CHD4) mRNA expression higher than the median copies had significantly shorter time to recurrence than those with lower than the median copies. Multivariate analysis showed that VEGF-A mRNA, CHD4 mRNA and lymphatic vessel involvement were independent prognostic factors for disease recurrence.
CONCLUSIONS: VEGF-A mRNA and CHD4 mRNA were up-regulated in CK20-positive pN0 lymph nodes and they may have prognostic significance in pN0 CRC patients.
METHODS: Two cohorts of lymph node specimens from pN0 CRC patients of each with and without CK20-positive cells were recruited. In the first cohort, tumor metastasis genes were profiled using gene expression arrays. Differential expressed genes were validated in the second cohort. Moreover, their prognostic significance was examined by following-up the second cohort of patients with CK20-positive cells for 60 months and all histopathological findings were correlated to recurrence.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
CHD4Humancolorectal cancer exacerbatesIEP mRNA:increased expression:lymph node (human)RGD 
Chd4Ratcolorectal cancer exacerbatesISOCHD4 (Homo sapiens)mRNA:increased expression:lymph node (human)RGD 
Chd4Mousecolorectal cancer exacerbatesISOCHD4 (Homo sapiens)mRNA:increased expression:lymph node (human)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Chd4  (chromodomain helicase DNA binding protein 4)

Genes (Mus musculus)
Chd4  (chromodomain helicase DNA binding protein 4)

Genes (Homo sapiens)
CHD4  (chromodomain helicase DNA binding protein 4)


Additional Information