RGD Reference Report - Liprin-a2 promotes the presynaptic recruitment and turnover of RIM1/CASK to facilitate synaptic transmission. - Rat Genome Database

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Liprin-a2 promotes the presynaptic recruitment and turnover of RIM1/CASK to facilitate synaptic transmission.

Authors: Spangler, Samantha A  Schmitz, Sabine K  Kevenaar, Josta T  de Graaff, Esther  de Wit, Heidi  Demmers, Jeroen  Toonen, Ruud F  Hoogenraad, Casper C 
Citation: Spangler SA, etal., J Cell Biol. 2013 Jun 10;201(6):915-28. doi: 10.1083/jcb.201301011.
RGD ID: 13702233
Pubmed: PMID:23751498   (View Abstract at PubMed)
PMCID: PMC3678157   (View Article at PubMed Central)
DOI: DOI:10.1083/jcb.201301011   (Journal Full-text)

The presynaptic active zone mediates synaptic vesicle exocytosis, and modulation of its molecular composition is important for many types of synaptic plasticity. Here, we identify synaptic scaffold protein liprin-α2 as a key organizer in this process. We show that liprin-α2 levels were regulated by synaptic activity and the ubiquitin-proteasome system. Furthermore, liprin-α2 organized presynaptic ultrastructure and controlled synaptic output by regulating synaptic vesicle pool size. The presence of liprin-α2 at presynaptic sites did not depend on other active zone scaffolding proteins but was critical for recruitment of several components of the release machinery, including RIM1 and CASK. Fluorescence recovery after photobleaching showed that depletion of liprin-α2 resulted in reduced turnover of RIM1 and CASK at presynaptic terminals, suggesting that liprin-α2 promotes dynamic scaffolding for molecular complexes that facilitate synaptic vesicle release. Therefore, liprin-α2 plays an important role in maintaining active zone dynamics to modulate synaptic efficacy in response to changes in network activity.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Ppfia2Ratregulation of synaptic vesicle exocytosis involved_inIDA PMID:23751498SynGO 
Ppfia2Ratregulation of synaptic vesicle exocytosis involved_inIMP PMID:23751498SynGO 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Ppfia2Ratglutamatergic synapse is_active_inIDA PMID:23751498SynGO 
Ppfia2Ratglutamatergic synapse is_active_inIMP PMID:23751498SynGO 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Ppfia2Ratstructural constituent of presynapse enablesIDA PMID:23751498SynGO 
Ppfia2Ratstructural constituent of presynapse enablesIMP PMID:23751498SynGO 

Objects Annotated

Genes (Rattus norvegicus)
Ppfia2  (PTPRF interacting protein alpha 2)


Additional Information