RGD Reference Report - Beclin-phosphatidylinositol 3-kinase complex functions at the trans-Golgi network. - Rat Genome Database

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Beclin-phosphatidylinositol 3-kinase complex functions at the trans-Golgi network.

Authors: Kihara, A  Kabeya, Y  Ohsumi, Y  Yoshimori, T 
Citation: Kihara A, etal., EMBO Rep. 2001 Apr;2(4):330-5.
RGD ID: 11561931
Pubmed: PMID:11306555   (View Abstract at PubMed)
PMCID: PMC1083858   (View Article at PubMed Central)
DOI: DOI:10.1093/embo-reports/kve061   (Journal Full-text)

Autophagy is an intracellular bulk protein degradation system. Beclin is known to be involved in this process; however, its role is unclear. In this study, we showed that Beclin was co-immunoprecipitated with phosphatidylinositol (PtdIns) 3-kinase, which is also required for autophagy, suggesting that Beclin is a component of the PtdIns 3-kinase complex. Quantitative analyses using a cross-linker showed that all Beclin forms a complex with PtdIns 3-kinase, whereas approximately 50% of PtdIns 3-kinase remains free from Beclin. Indirect immunofluorescence microscopy demonstrated that the majority of Beclin and PtdIns 3-kinase localize to the trans-Golgi network (TGN). Some PtdIns 3-kinase is also distributed in the late endosome. These results suggest that Beclin and PtdIns 3-kinase control autophagy as a complex at the TGN.




Cellular Component

  
Object Symbol
Species
Term
Qualifier
Evidence
With
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Original Reference(s)
Becn1Rattrans-Golgi network located_inIDA  RGD 


Genes (Rattus norvegicus)
Becn1  (beclin 1)