RGD Reference Report - Role of CIP4 in high glucose induced epithelial--mesenchymal transition of rat peritoneal mesothelial cells. - Rat Genome Database

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Role of CIP4 in high glucose induced epithelial--mesenchymal transition of rat peritoneal mesothelial cells.

Authors: Zhang, J  Bi, M  Zhong, F  Jiao, X  Zhang, D  Dong, Q 
Citation: Zhang J, etal., Ren Fail. 2013 Aug;35(7):989-95. doi: 10.3109/0886022X.2013.808957. Epub 2013 Jul 2.
RGD ID: 11535143
Pubmed: PMID:23819628   (View Abstract at PubMed)
DOI: DOI:10.3109/0886022X.2013.808957   (Journal Full-text)

BACKGROUND: Peritoneal mesothelial cell (PMC) plays a key role in the process of peritoneal fibrosis. Epithelial-mesenchymal transition (EMT) of PMCs is an important mechanism of peritoneal fibrosis. Prolonged exposure to peritoneal dialysis fluid containing a high concentration of glucose may lead to EMT of PMCs. Cdc42-interacting protein-4 (CIP4) is a critical regulator of cell skeleton and downstream effector of Cdc42 and participates in EMT of tubular epithelial cells. In the present study, we investigate the possible role of CIP4 in EMT of PMC under high glucose (HG) condition in vitro and further explore the potential therapeutic point for peritoneal fibrosis. METHODS: Rat peritoneal mesothelial cells (RPMCs) were isolated from the peritonea of rats by enzymatic digestion. Under HG conditions (1.5%, 2.5% and 4.25%), E-cadherin, alpha-SMA and CIP4 expression were assessed by Western blot. Effect of CIP4-siRNA and pcDNA3.1-CIP4 transfection on E-cadherin, alpha-SMA and CIP4 expression were also assessed respectively under 2.5% HG concentration. Cells were pretreated for 24 h with PI3K/Akt signaling inhibitor perifosine and effect of perifosine on CIP4 expression were detected by Western blot. RESULTS: EMT induction by HG was confirmed by the prevalence of morphological changes, loss of E-cadherin, increase in alpha-SMA expression. CIP4-siRNA transfection can reverse EMT of RPMCs. Over-expression of CIP4 promoted characteristics similar to those commonly observed in EMT. Furthermore, the increased CIP4 in response to HG was efficiently inhibited by perifosine. CONCLUSION: This study shows that CIP4 promotes high glucose-induced EMT through PI3K-Akt signaling pathway in RPMCs.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
epithelial to mesenchymal transition  IMP 11535143 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Trip10  (thyroid hormone receptor interactor 10)


Additional Information