RGD Reference Report - Regulation of AMPA receptor channels and synaptic plasticity by cofilin phosphatase Slingshot in cortical neurons. - Rat Genome Database

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Regulation of AMPA receptor channels and synaptic plasticity by cofilin phosphatase Slingshot in cortical neurons.

Authors: Yuen, EY  Liu, W  Kafri, T  Van Praag, H  Yan, Z 
Citation: Yuen EY, etal., J Physiol. 2010 Jul 1;588(Pt 13):2361-71. doi: 10.1113/jphysiol.2009.186353. Epub 2010 May 4.
RGD ID: 11535133
Pubmed: (View Article at PubMed) PMID:20442266
DOI: Full-text: DOI:10.1113/jphysiol.2009.186353

Cofilin, the major actin depolymerizing factor, modulates actin dynamics that contribute to spine morphology, synaptic transmission and plasticity. Much evidence implicates the cofilin inactivation kinase LIMK in synaptic function, but little is known about the cofilin activation phosphatase Slingshot in this regard. In this study, we found that suppressing endogenous Slingshot with small RNA interference or function-blocking antibody led to a dramatic reduction of AMPA receptor-mediated excitatory postsynaptic currents (EPSCs) in cortical neurons. Perturbation of Slingshot function also diminished the ability to express synaptic plasticity. Inactivating cofilin or disturbing actin dynamics reduced AMPAR-EPSCs in a Slingshot-dependent manner. Moreover, surface GluR 1 and synaptic GluR2/3 clusters were reduced by Slingshot knockdown. Our data suggest that Slingshot plays a pivotal role in AMPAR trafficking and synaptic transmission by controlling actin cytoskeleton via cofilin activation.

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Genes (Rattus norvegicus)
Ssh1  (slingshot protein phosphatase 1)


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