RGD Reference Report - Ribosomal protein L30 is a component of the UGA-selenocysteine recoding machinery in eukaryotes. - Rat Genome Database

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Ribosomal protein L30 is a component of the UGA-selenocysteine recoding machinery in eukaryotes.

Authors: Chavatte, L  Brown, BA  Driscoll, DM 
Citation: Chavatte L, etal., Nat Struct Mol Biol. 2005 May;12(5):408-16. Epub 2005 Apr 10.
RGD ID: 11038818
Pubmed: PMID:15821744   (View Abstract at PubMed)
DOI: DOI:10.1038/nsmb922   (Journal Full-text)

The translational recoding of UGA as selenocysteine (Sec) is directed by a SECIS element in the 3' untranslated region (UTR) of eukaryotic selenoprotein mRNAs. The selenocysteine insertion sequence (SECIS) contains two essential tandem sheared G.A pairs that bind SECIS-binding protein 2 (SBP2), which recruits a selenocysteine-specific elongation factor and Sec-tRNA(Sec) to the ribosome. Here we show that ribosomal protein L30 is a component of the eukaryotic selenocysteine recoding machinery. L30 binds SECIS elements in vitro and in vivo, stimulates UGA recoding in transfected cells and competes with SBP2 for SECIS binding. Magnesium, known to induce a kink-turn in RNAs that contain two tandem G.A pairs, decreases the SBP2-SECIS complex in favor of the L30-SECIS interaction. We propose a model in which SBP2 and L30 carry out different functions in the UGA recoding mechanism, with the SECIS acting as a molecular switch upon protein binding.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Rpl30Ratpositive regulation of selenocysteine incorporation  IDA  RGD 
Secisbp2Ratpositive regulation of selenocysteine incorporation  IDA  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Rpl30Ratselenocysteine insertion sequence binding  IDA  RGD 
Secisbp2Ratselenocysteine insertion sequence binding  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Rpl30  (ribosomal protein L30)
Secisbp2  (SECIS binding protein 2)


Additional Information