Dhfr (dihydrofolate reductase) - Rat Genome Database

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Gene: Dhfr (dihydrofolate reductase) Rattus norvegicus
Analyze
Symbol: Dhfr
Name: dihydrofolate reductase
RGD ID: 2500
Description: Enables NADP binding activity; dihydrofolate reductase activity; and dihydrofolic acid binding activity. Involved in several processes, including axon regeneration; dicarboxylic acid metabolic process; and tetrahydrofolate biosynthetic process. Located in mitochondrion. Human ortholog(s) of this gene implicated in acute lymphoblastic leukemia. Orthologous to several human genes including DHFR (dihydrofolate reductase); PARTICIPATES IN folate mediated one-carbon metabolic pathway; folate metabolic pathway; pyrimidine metabolic pathway; INTERACTS WITH (+)-schisandrin B; 2,3,7,8-tetrachlorodibenzodioxine; 6-propyl-2-thiouracil.
Type: protein-coding
RefSeq Status: PROVISIONAL
Previously known as: Dhfr1; Dihydrofolate reductase 1 (active)
RGD Orthologs
Human
Mouse
Chinchilla
Bonobo
Dog
Squirrel
Pig
Green Monkey
Naked Mole-Rat
Black Rat
Alliance Orthologs
More Info more info ...
Latest Assembly: GRCr8 - GRCr8 Assembly
Position:
Rat AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
GRCr8225,320,895 - 25,346,004 (+)NCBIGRCr8GRCr8GRCr8
mRatBN7.2223,585,876 - 23,611,199 (+)NCBImRatBN7.2mRatBN7.2
mRatBN7.2 Ensembl223,586,031 - 23,613,713 (+)EnsemblmRatBN7.2
UTH_Rnor_SHR_Utx230,620,446 - 30,644,695 (+)NCBIUTH_Rnor_SHR_UtxUTH_Rnor_SHR_Utx
UTH_Rnor_SHRSP_BbbUtx_1.0228,720,807 - 28,745,052 (+)NCBIUTH_Rnor_SHRSP_BbbUtx_1.0UTH_Rnor_SHRSP_BbbUtx_1.0
UTH_Rnor_WKY_Bbb_1.0223,548,630 - 23,573,162 (+)NCBIUTH_Rnor_WKY_Bbb_1.0UTH_Rnor_WKY_Bbb_1.0
Rnor_6.0221,931,887 - 21,958,927 (+)NCBIRnor_6.0Rnor_6.0rn6Rnor6.0
Rnor_6.0 Ensembl221,931,493 - 21,962,453 (+)Ensemblrn6Rnor6.0
Rnor_5.0241,162,308 - 41,162,496 (+)NCBIRnor_5.0Rnor_5.0rn5
Rnor_5.0241,137,784 - 41,149,417 (+)NCBIRnor_5.0Rnor_5.0rn5
RGSC_v3.4222,607,093 - 22,828,487 (+)NCBIRGSC_v3.4RGSC_v3.4rn4
Celera219,675,965 - 19,700,103 (+)NCBICelera
RGSC_v3.1222,527,461 - 22,748,856 (+)NCBI
Cytogenetic Map2q12NCBI
JBrowse:




Gene-Chemical Interaction Annotations     Click to see Annotation Detail View
(+)-epicatechin-3-O-gallate  (ISO)
(+)-schisandrin B  (EXP)
(-)-demecolcine  (ISO)
(-)-epigallocatechin 3-gallate  (ISO)
(S)-nicotine  (ISO)
1,1-dichloroethene  (ISO)
1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine  (ISO)
14-Deoxy-11,12-didehydroandrographolide  (ISO)
17alpha-ethynylestradiol  (ISO)
17beta-estradiol  (ISO)
2,2',4,4',5,5'-hexachlorobiphenyl  (ISO)
2,3,7,8-tetrachlorodibenzodioxine  (EXP,ISO)
3,3',4,4'-tetrachlorobiphenyl  (ISO)
3-methylcholanthrene  (ISO)
4,4'-sulfonyldiphenol  (ISO)
4-hydroxyphenyl retinamide  (ISO)
5-fluorouracil  (ISO)
5-formyltetrahydrofolic acid  (ISO)
6-bromoindirubin-3'-oxime  (ISO)
6-propyl-2-thiouracil  (EXP)
acrylamide  (ISO)
aflatoxin B1  (ISO)
aflatoxin M1  (ISO)
all-trans-retinoic acid  (ISO)
amitrole  (EXP)
ammonium chloride  (EXP)
aristolochic acid A  (ISO)
arsane  (ISO)
arsenic atom  (ISO)
arsenite(3-)  (ISO)
benzo[a]pyrene  (ISO)
benzo[a]pyrene diol epoxide I  (ISO)
benzo[e]pyrene  (ISO)
beta-carotene  (ISO)
bis(2-ethylhexyl) phthalate  (ISO)
bisphenol A  (EXP,ISO)
cadmium atom  (EXP)
cadmium dichloride  (EXP)
captan  (ISO)
carbamazepine  (ISO)
carbon nanotube  (ISO)
cefazolin  (ISO)
cefepime  (ISO)
ceftazidime pentahydrate  (ISO)
ceftizoxime  (ISO)
ceftriaxone  (ISO)
chlorambucil  (ISO)
choline  (ISO)
chromium(6+)  (ISO)
cisplatin  (ISO)
clobetasol  (ISO)
cobalt dichloride  (ISO)
copper atom  (ISO)
copper(0)  (ISO)
copper(II) sulfate  (ISO)
coumestrol  (ISO)
cyclosporin A  (ISO)
cytarabine  (ISO)
DDE  (ISO)
diallyl trisulfide  (ISO)
dibenzo[a,l]pyrene  (ISO)
Dibutyl phosphate  (ISO)
dibutyl phthalate  (ISO)
diclofenac  (ISO)
dimethyl sulfoxide  (ISO)
DISTAMYCIN A  (ISO)
doxorubicin  (ISO)
emodin  (ISO)
endosulfan  (EXP)
Enterolactone  (ISO)
ethanol  (ISO)
ferulic acid  (ISO)
folic acid  (EXP,ISO)
folpet  (ISO)
formaldehyde  (ISO)
fulvestrant  (ISO)
genistein  (ISO)
gentamycin  (EXP)
glyphosate  (EXP)
GSK-J4  (ISO)
indometacin  (EXP)
inulin  (ISO)
isoflavones  (ISO)
isotretinoin  (ISO)
ivermectin  (ISO)
L-methionine  (ISO)
lamivudine  (ISO)
leflunomide  (EXP)
levofloxacin  (ISO)
Mecamylamine  (ISO)
methapyrilene  (ISO)
methimazole  (EXP)
methotrexate  (EXP,ISO)
methylmercury chloride  (ISO)
methylparaben  (ISO)
methylseleninic acid  (ISO)
N-acetyl-L-cysteine  (ISO)
N-methyl-4-phenylpyridinium  (ISO)
N-nitrosodiethylamine  (EXP)
N-Nitrosopyrrolidine  (ISO)
NADP zwitterion  (ISO)
NADP(+)  (ISO)
naringin  (ISO)
nickel atom  (ISO)
niclosamide  (ISO)
nicotine  (ISO)
nimesulide  (EXP)
nitrogen mustard  (ISO)
olmesartan  (EXP)
oxaliplatin  (EXP)
ozone  (ISO)
paracetamol  (EXP,ISO)
pemetrexed  (ISO)
perfluorohexanesulfonic acid  (ISO)
perfluorooctane-1-sulfonic acid  (ISO)
perfluorooctanoic acid  (ISO)
phenethyl caffeate  (EXP)
phenobarbital  (ISO)
pirinixic acid  (ISO)
piroxicam  (ISO)
potassium chromate  (ISO)
potassium dichromate  (ISO)
propanal  (ISO)
propiconazole  (EXP)
pyrimethamine  (ISO)
Rebamipide  (EXP)
resveratrol  (ISO)
rotenone  (EXP)
silicon dioxide  (ISO)
sodium arsenate  (ISO)
sodium arsenite  (EXP,ISO)
sulfadimethoxine  (EXP)
sunitinib  (ISO)
syringic acid  (ISO)
tamoxifen  (ISO)
tetrachloromethane  (EXP,ISO)
thioacetamide  (EXP)
thiram  (ISO)
topotecan  (EXP)
trabectedin  (ISO)
trimellitic anhydride  (ISO)
trimethoprim  (ISO)
trimetrexate  (ISO)
triphenyl phosphate  (ISO)
triptonide  (ISO)
tubocurarine  (ISO)
urethane  (ISO)
valproic acid  (ISO)
vincristine  (ISO)
vorinostat  (ISO)
zidovudine  (ISO)

Gene Ontology Annotations     Click to see Annotation Detail View

Cellular Component
cytoplasm  (IDA,IEA,ISO)
cytosol  (ISO)
mitochondrion  (IBA,IDA,IEA,ISO)
nucleus  (IEA,ISO)
replication fork  (ISO)

References

References - curated
# Reference Title Reference Citation
1. DNA variants in region for noncoding interfering transcript of dihydrofolate reductase gene and outcome in childhood acute lymphoblastic leukemia. Al-Shakfa F, etal., Clin Cancer Res. 2009 Nov 15;15(22):6931-8. doi: 10.1158/1078-0432.CCR-09-0641. Epub 2009 Oct 27.
2. Dihydrofolate reductase deficiency due to a homozygous DHFR mutation causes megaloblastic anemia and cerebral folate deficiency leading to severe neurologic disease. Cario H, etal., Am J Hum Genet. 2011 Feb 11;88(2):226-31. doi: 10.1016/j.ajhg.2011.01.007.
3. Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Gaudet P, etal., Brief Bioinform. 2011 Sep;12(5):449-62. doi: 10.1093/bib/bbr042. Epub 2011 Aug 27.
4. Transport, binding, and polyglutamation of methotrexate in freshly isolated rat hepatocytes. Gewirtz DA, etal., Cancer Res. 1980 Mar;40(3):573-8.
5. Rat ISS GO annotations from GOA human gene data--August 2006 GOA data from the GO Consortium
6. Survival and risk of relapse of acute lymphoblastic leukemia in a Mexican population is affected by dihydrofolate reductase gene polymorphisms. Gomez-Gomez Y, etal., Exp Ther Med. 2012 Apr;3(4):665-672. Epub 2012 Jan 9.
7. Persistent nicotine treatment potentiates amplification of the dihydrofolate reductase gene in rat lung epithelial cells as a consequence of Ras activation. Guo J, etal., J Biol Chem. 2005 Aug 26;280(34):30422-31. Epub 2005 Jun 27.
8. An active second dihydrofolate reductase enzyme is not a feature of rat and mouse, but they do have activity in their mitochondria. Hughes L, etal., FEBS Lett. 2015 Jul 8;589(15):1855-62. doi: 10.1016/j.febslet.2015.05.017. Epub 2015 May 14.
9. Folate regulation of axonal regeneration in the rodent central nervous system through DNA methylation. Iskandar BJ, etal., J Clin Invest. 2010 May;120(5):1603-16. doi: 10.1172/JCI40000. Epub 2010 Apr 26.
10. Reduced folate carrier and dihydrofolate reductase expression in acute lymphocytic leukemia may predict outcome: a Children's Cancer Group Study. Levy AS, etal., J Pediatr Hematol Oncol. 2003 Sep;25(9):688-95.
11. Permissivity for methotrexate-induced DHFR gene amplification correlates with the metastatic potential of rat adenocarcinoma cells. Lucke-Huhle C Carcinogenesis. 1994 Apr;15(4):695-700.
12. Increased frequency of expression of elevated dihydrofolate reductase in T-cell versus B-precursor acute lymphoblastic leukemia in children. Matherly LH, etal., Blood. 1997 Jul 15;90(2):578-89.
13. Rat ISS GO annotations from MGI mouse gene data--August 2006 MGD data from the GO Consortium
14. Electronic Transfer of LocusLink and RefSeq Data NCBI rat LocusLink and RefSeq merged data July 26, 2002
15. OMIM Disease Annotation Pipeline OMIM Disease Annotation Pipeline
16. KEGG Annotation Import Pipeline Pipeline to import KEGG annotations from KEGG into RGD
17. SMPDB Annotation Import Pipeline Pipeline to import SMPDB annotations from SMPDB into RGD
18. GOA pipeline RGD automated data pipeline
19. ClinVar Automated Import and Annotation Pipeline RGD automated import pipeline for ClinVar variants, variant-to-disease annotations and gene-to-disease annotations
20. Data Import for Chemical-Gene Interactions RGD automated import pipeline for gene-chemical interactions
21. Comprehensive gene review and curation RGD comprehensive gene curation
22. Role of angiotensin II on dihydrofolate reductase, GTP-cyclohydrolase 1 and nitric oxide synthase expressions in renal ischemia-reperfusion. Seujange Y, etal., Am J Nephrol. 2008;28(4):692-700. doi: 10.1159/000126927. Epub 2008 Apr 11.
23. Trafficking of intracellular folates. Stover PJ and Field MS, Adv Nutr. 2011 Jul;2(4):325-31. doi: 10.3945/¿an.111.000596. Epub 2011 Jun 28.
24. Trimetrexate inhibits progression of the murine 32Dp210 model of chronic myeloid leukemia in animals expressing drug-resistant dihydrofolate reductase. Sweeney CL, etal., Cancer Res. 2003 Mar 15;63(6):1304-10.
25. Isolation of rat dihydrofolate reductase gene and characterization of recombinant enzyme. Wang Y, etal., Antimicrob Agents Chemother 2001 Sep;45(9):2517-23.
26. Update and new concepts in vitamin responsive disorders of folate transport and metabolism. Watkins D and Rosenblatt DS, J Inherit Metab Dis. 2012 Jul;35(4):665-70. doi: 10.1007/s10545-011-9418-1. Epub 2011 Nov 23.
27. Comparative activity of rat liver dihydrofolate reductase with 7,8-dihydrofolate and other 7,8-dihydropteridines. Webber S and Whiteley JM, Arch Biochem Biophys. 1985 Feb 1;236(2):681-90.
Additional References at PubMed
PMID:2303423   PMID:8490020   PMID:12096917   PMID:12477932   PMID:14667810   PMID:15039552   PMID:15974594   PMID:19666465   PMID:21876184   PMID:23707606  


Genomics

Comparative Map Data
Dhfr
(Rattus norvegicus - Norway rat)
Rat AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
GRCr8225,320,895 - 25,346,004 (+)NCBIGRCr8GRCr8GRCr8
mRatBN7.2223,585,876 - 23,611,199 (+)NCBImRatBN7.2mRatBN7.2
mRatBN7.2 Ensembl223,586,031 - 23,613,713 (+)EnsemblmRatBN7.2
UTH_Rnor_SHR_Utx230,620,446 - 30,644,695 (+)NCBIUTH_Rnor_SHR_UtxUTH_Rnor_SHR_Utx
UTH_Rnor_SHRSP_BbbUtx_1.0228,720,807 - 28,745,052 (+)NCBIUTH_Rnor_SHRSP_BbbUtx_1.0UTH_Rnor_SHRSP_BbbUtx_1.0
UTH_Rnor_WKY_Bbb_1.0223,548,630 - 23,573,162 (+)NCBIUTH_Rnor_WKY_Bbb_1.0UTH_Rnor_WKY_Bbb_1.0
Rnor_6.0221,931,887 - 21,958,927 (+)NCBIRnor_6.0Rnor_6.0rn6Rnor6.0
Rnor_6.0 Ensembl221,931,493 - 21,962,453 (+)Ensemblrn6Rnor6.0
Rnor_5.0241,162,308 - 41,162,496 (+)NCBIRnor_5.0Rnor_5.0rn5
Rnor_5.0241,137,784 - 41,149,417 (+)NCBIRnor_5.0Rnor_5.0rn5
RGSC_v3.4222,607,093 - 22,828,487 (+)NCBIRGSC_v3.4RGSC_v3.4rn4
Celera219,675,965 - 19,700,103 (+)NCBICelera
RGSC_v3.1222,527,461 - 22,748,856 (+)NCBI
Cytogenetic Map2q12NCBI
DHFR
(Homo sapiens - human)
Human AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
GRCh38580,626,226 - 80,654,983 (-)NCBIGRCh38GRCh38hg38GRCh38
GRCh38.p14 Ensembl580,626,226 - 80,654,983 (-)Ensemblhg38GRCh38
GRCh37579,922,045 - 79,950,802 (-)NCBIGRCh37GRCh37hg19GRCh37
Build 36579,957,801 - 79,986,556 (-)NCBIBuild 36Build 36hg18NCBI36
Build 34579,957,803 - 79,986,572NCBI
Celera575,825,049 - 75,854,857 (-)NCBICelera
Cytogenetic Map5q14.1NCBI
HuRef575,130,997 - 75,159,721 (-)NCBIHuRef
CHM1_1579,355,281 - 79,384,046 (-)NCBICHM1_1
T2T-CHM13v2.0581,111,116 - 81,139,892 (-)NCBIT2T-CHM13v2.0
Dhfr
(Mus musculus - house mouse)
Mouse AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
GRCm391392,491,291 - 92,525,561 (+)NCBIGRCm39GRCm39mm39
GRCm39 Ensembl1392,491,234 - 92,525,561 (+)EnsemblGRCm39 EnsemblGRCm39
GRCm381392,354,783 - 92,389,053 (+)NCBIGRCm38GRCm38mm10GRCm38
GRCm38.p6 Ensembl1392,354,726 - 92,389,053 (+)Ensemblmm10GRCm38
MGSCv371393,124,738 - 93,159,008 (+)NCBIMGSCv37MGSCv37mm9NCBIm37
MGSCv361393,455,536 - 93,489,806 (+)NCBIMGSCv36mm8
Celera1395,961,012 - 95,994,907 (+)NCBICelera
Cytogenetic Map13C3NCBI
cM Map1347.64NCBI
Dhfr
(Chinchilla lanigera - long-tailed chinchilla)
Chinchilla AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
ChiLan1.0 EnsemblNW_00495541829,274,170 - 29,283,028 (+)Ensembl
ChiLan1.0NW_00495541829,274,170 - 29,283,028 (+)NCBIChiLan1.0ChiLan1.0
DHFR
(Pan paniscus - bonobo/pygmy chimpanzee)
Bonobo AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
NHGRI_mPanPan1-v2434,653,369 - 34,681,917 (+)NCBINHGRI_mPanPan1-v2
NHGRI_mPanPan1532,806,994 - 32,835,542 (+)NCBINHGRI_mPanPan1
Mhudiblu_PPA_v0534,663,210 - 34,689,467 (+)NCBIMhudiblu_PPA_v0Mhudiblu_PPA_v0panPan3
PanPan1.1534,938,548 - 34,967,003 (+)NCBIPanPan1.1PanPan1.1panPan2
PanPan1.1 Ensembl534,938,548 - 34,967,003 (+)EnsemblpanPan2panpan1.1
DHFR
(Canis lupus familiaris - dog)
Dog AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
CanFam3.1326,521,214 - 26,556,065 (+)NCBICanFam3.1CanFam3.1canFam3CanFam3.1
Dog10K_Boxer_Tasha327,762,458 - 27,797,614 (+)NCBIDog10K_Boxer_TashaDog10K_Boxer_Tasha
ROS_Cfam_1.0326,441,764 - 26,476,948 (+)NCBIROS_Cfam_1.0ROS_Cfam_1.0
ROS_Cfam_1.0 Ensembl326,441,755 - 26,468,894 (+)EnsemblROS_Cfam_1.0 Ensembl
UMICH_Zoey_3.1326,381,985 - 26,417,256 (+)NCBIUMICH_Zoey_3.1
UNSW_CanFamBas_1.0326,356,657 - 26,413,875 (+)NCBIUNSW_CanFamBas_1.0
UU_Cfam_GSD_1.0326,636,300 - 26,671,702 (+)NCBIUU_Cfam_GSD_1.0UU_Cfam_GSD_1.0
Dhfr
(Ictidomys tridecemlineatus - thirteen-lined ground squirrel)
Squirrel AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
HiC_Itri_2NW_024407213182,542,580 - 182,561,929 (+)NCBIHiC_Itri_2
SpeTri2.0 EnsemblNW_00493646961,990 - 83,553 (-)EnsemblSpeTri2.0 Ensembl
SpeTri2.0NW_00493646967,962 - 83,116 (-)NCBISpeTri2.0SpeTri2.0SpeTri2.0
DHFR
(Sus scrofa - pig)
Pig AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
Sscrofa11.1289,230,660 - 89,255,830 (-)NCBISscrofa11.1Sscrofa11.1susScr11Sscrofa11.1
Sscrofa10.2291,077,865 - 91,102,076 (-)NCBISscrofa10.2Sscrofa10.2susScr3
DHFR
(Chlorocebus sabaeus - green monkey)
Green Monkey AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
ChlSab1.1474,787,292 - 74,811,196 (-)NCBIChlSab1.1ChlSab1.1chlSab2
ChlSab1.1 Ensembl474,787,629 - 74,810,960 (-)EnsemblChlSab1.1 EnsemblchlSab2Vervet-AGM
Vero_WHO_p1.0NW_02366604923,650,277 - 23,674,290 (-)NCBIVero_WHO_p1.0Vero_WHO_p1.0
Dhfr
(Heterocephalus glaber - naked mole-rat)
Naked Mole-Rat AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
HetGla_female_1.0 EnsemblNW_0046247431,446,643 - 1,461,863 (-)EnsemblHetGla_female_1.0 EnsemblhetGla2HetGla_female_1.0 Ensembl
HetGla 1.0NW_0046247431,446,643 - 1,462,319 (-)NCBIHetGla 1.0HetGla 1.0hetGla2
Dhfr
(Rattus rattus - black rat)
Black Rat AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
Rrattus_CSIRO_v13203,914,132 - 203,936,441 (-)NCBIRrattus_CSIRO_v1

Variants

.
Variants in Dhfr
291 total Variants
miRNA Target Status (No longer updated)

Predicted Target Of
Summary Value
Count of predictions:91
Count of miRNA genes:72
Interacting mature miRNAs:83
Transcripts:ENSRNOT00000018259
Prediction methods:Miranda, Rnahybrid, Targetscan
Result types:miRGate_prediction

The detailed report is available here: Full Report CSV TAB Printer

miRNA Target Status data imported from miRGate (http://mirgate.bioinfo.cnio.es/).
For more information about miRGate, see PMID:25858286 or access the full paper here.


QTLs in Region (GRCr8)
The following QTLs overlap with this region.    Full Report CSV TAB Printer Gviewer
RGD IDSymbolNameLODP ValueTraitSub TraitChrStartStopSpecies
1331764Bp205Blood pressure QTL 2053.476arterial blood pressure trait (VT:2000000)mean arterial blood pressure (CMO:0000009)21436870759368707Rat
10755430Coatc6Coat color QTL 60.02576coat/hair pigmentation trait (VT:0010463)pigmented ventral coat/hair area to total ventral coat/hair area ratio (CMO:0001812)21332504158325041Rat
1600379Mcs18Mammary carcinoma susceptibility QTL 182.6mammary gland integrity trait (VT:0010552)mammary tumor number (CMO:0000343)2962358144538110Rat
1331760Bp206Blood pressure QTL 2063.62454arterial blood pressure trait (VT:2000000)mean arterial blood pressure (CMO:0000009)24265106205135428Rat
7387318Stl32Serum triglyceride level QTL 323.20.0003blood triglyceride amount (VT:0002644)plasma triglyceride level (CMO:0000548)22411781369117813Rat
61355Bp36Blood pressure QTL 362.9blood pressure trait (VT:0000183)systolic blood pressure (CMO:0000004)27605533104774005Rat
10755499Bp389Blood pressure QTL 3892.61arterial blood pressure trait (VT:2000000)diastolic blood pressure (CMO:0000005)220695736231474293Rat
1578664Bmd9Bone mineral QTL density 95femur mineral mass (VT:0010011)total volumetric bone mineral density (CMO:0001728)2573632550736325Rat
738010Lnnr3Liver neoplastic nodule remodeling QTL 32.94liver integrity trait (VT:0010547)liver remodeling tumorous lesion number (CMO:0001461)2142977529Rat
9590080Insglur4Insulin/glucose ratio QTL 428.70.001blood insulin amount (VT:0001560)calculated plasma insulin level (CMO:0002170)2615276751152767Rat
1579916Bp270Blood pressure QTL 2700.003arterial blood pressure trait (VT:2000000)mean arterial blood pressure (CMO:0000009)21605188361051883Rat
731167Glom4Glomerulus QTL 42.40.0082kidney glomerulus morphology trait (VT:0005325)count of superficial glomeruli not directly contacting the kidney surface (CMO:0001002)22203804467038044Rat
731166Mamtr2Mammary tumor resistance QTL 20.0001mammary gland integrity trait (VT:0010552)mammary tumor number (CMO:0000343)2962358154623581Rat
731184Mamtr4Mammary tumor resistance QTL 40.0003mammary gland integrity trait (VT:0010552)mammary tumor number (CMO:0000343)21822713763227137Rat

Markers in Region
BE121398  
Rat AssemblyChrPosition (strand)SourceJBrowse
mRatBN7.2223,589,098 - 23,589,247 (+)MAPPERmRatBN7.2
Rnor_6.0221,934,990 - 21,935,138NCBIRnor6.0
Rnor_5.0241,140,887 - 41,141,035UniSTSRnor5.0
RGSC_v3.4222,610,196 - 22,610,344UniSTSRGSC3.4
Celera219,679,068 - 19,679,216UniSTS
Cytogenetic Map2q12UniSTS
RH128352  
Rat AssemblyChrPosition (strand)SourceJBrowse
mRatBN7.2223,674,479 - 23,674,674 (+)MAPPERmRatBN7.2
Rnor_6.0222,023,201 - 22,023,395NCBIRnor6.0
Rnor_5.0241,226,672 - 41,226,866UniSTSRnor5.0
RGSC_v3.4222,686,665 - 22,686,859UniSTSRGSC3.4
Celera219,764,314 - 19,764,508UniSTS
Cytogenetic Map2q12UniSTS
RH133371  
Rat AssemblyChrPosition (strand)SourceJBrowse
Cytogenetic Map2q12UniSTS
RH134136  
Rat AssemblyChrPosition (strand)SourceJBrowse
mRatBN7.2223,610,504 - 23,610,696 (+)MAPPERmRatBN7.2
Rnor_6.0221,959,240 - 21,959,431NCBIRnor6.0
Rnor_5.0241,162,809 - 41,163,000UniSTSRnor5.0
RGSC_v3.4222,828,800 - 22,828,991UniSTSRGSC3.4
RGSC_v3.44415,160 - 415,342UniSTSRGSC3.4
Celera219,700,416 - 19,700,607UniSTS
Cytogenetic Map2q12UniSTS
AW555094  
Rat AssemblyChrPosition (strand)SourceJBrowse
mRatBN7.245,173,854 - 5,173,937 (-)MAPPERmRatBN7.2
mRatBN7.2223,610,614 - 23,610,697 (+)MAPPERmRatBN7.2
Rnor_6.0221,959,350 - 21,959,432NCBIRnor6.0
Rnor_6.043,086,611 - 3,086,693NCBIRnor6.0
Rnor_5.043,139,041 - 3,139,123UniSTSRnor5.0
Rnor_5.0241,162,919 - 41,163,001UniSTSRnor5.0
RGSC_v3.4222,828,910 - 22,828,992UniSTSRGSC3.4
RGSC_v3.44415,262 - 415,344UniSTSRGSC3.4
Celera45,137,637 - 5,137,719UniSTS
Celera219,700,526 - 19,700,608UniSTS
Cytogenetic Map2q12UniSTS
BI302236  
Rat AssemblyChrPosition (strand)SourceJBrowse
mRatBN7.2223,805,580 - 23,805,730 (+)MAPPERmRatBN7.2
Rnor_6.0222,154,173 - 22,154,322NCBIRnor6.0
Rnor_5.0241,357,420 - 41,357,569UniSTSRnor5.0
RGSC_v3.4222,818,070 - 22,818,219UniSTSRGSC3.4
Celera219,894,943 - 19,895,092UniSTS
Cytogenetic Map2q12UniSTS
RH141201  
Rat AssemblyChrPosition (strand)SourceJBrowse
mRatBN7.2223,667,098 - 23,667,279 (+)MAPPERmRatBN7.2
Rnor_6.0222,015,822 - 22,016,002NCBIRnor6.0
Rnor_5.0241,219,463 - 41,219,643UniSTSRnor5.0
RGSC_v3.4222,678,763 - 22,678,941UniSTSRGSC3.4
Celera219,757,016 - 19,757,196UniSTS
Cytogenetic Map2q12UniSTS
RH141424  
Rat AssemblyChrPosition (strand)SourceJBrowse
mRatBN7.2223,757,556 - 23,757,761 (+)MAPPERmRatBN7.2
Rnor_6.0222,106,277 - 22,106,481NCBIRnor6.0
Rnor_5.0241,309,463 - 41,309,667UniSTSRnor5.0
RGSC_v3.4222,769,741 - 22,769,945UniSTSRGSC3.4
Celera219,847,396 - 19,847,600UniSTS
Cytogenetic Map2q12UniSTS


Expression

RNA-SEQ Expression

alimentary part of gastrointestinal system
appendage
circulatory system
ectoderm
endocrine system
endoderm
exocrine system
hemolymphoid system
hepatobiliary system
integumental system
mesenchyme
mesoderm
musculoskeletal system
nervous system
renal system
reproductive system
respiratory system
sensory system
visual system
16 12 67 165 91 90 59 92 59 6 356 192 11 144 81 92 31 17 17

Sequence


Ensembl Acc Id: ENSRNOT00000018259   ⟹   ENSRNOP00000018259
Type: CODING
Position:
Rat AssemblyChrPosition (strand)Source
mRatBN7.2 Ensembl223,586,031 - 23,613,713 (+)Ensembl
Rnor_6.0 Ensembl221,931,493 - 21,962,453 (+)Ensembl
RefSeq Acc Id: NM_130400   ⟹   NP_569084
RefSeq Status: PROVISIONAL
Type: CODING
Position:
Rat AssemblyChrPosition (strand)Source
GRCr8225,321,076 - 25,345,272 (+)NCBI
mRatBN7.2223,585,996 - 23,610,192 (+)NCBI
Rnor_6.0221,931,887 - 21,958,927 (+)NCBI
Rnor_5.0241,137,784 - 41,149,417 (+)NCBI
Rnor_5.0241,162,308 - 41,162,496 (+)NCBI
RGSC_v3.4222,607,093 - 22,828,487 (+)RGD
Celera219,675,965 - 19,700,103 (+)RGD
Sequence:
RefSeq Acc Id: XM_039101710   ⟹   XP_038957638
Type: CODING
Position:
Rat AssemblyChrPosition (strand)Source
GRCr8225,320,895 - 25,346,004 (+)NCBI
mRatBN7.2223,585,876 - 23,611,199 (+)NCBI
Protein Sequences
Protein RefSeqs NP_569084 (Get FASTA)   NCBI Sequence Viewer  
  XP_038957638 (Get FASTA)   NCBI Sequence Viewer  
GenBank Protein AAI58584 (Get FASTA)   NCBI Sequence Viewer  
  AAL11500 (Get FASTA)   NCBI Sequence Viewer  
  EDM10015 (Get FASTA)   NCBI Sequence Viewer  
Ensembl Protein ENSRNOP00000018259.3
  ENSRNOP00000109261
GenBank Protein Q920D2 (Get FASTA)   NCBI Sequence Viewer  
RefSeq Acc Id: NP_569084   ⟸   NM_130400
- UniProtKB: Q920D2 (UniProtKB/Swiss-Prot),   B0BMV8 (UniProtKB/TrEMBL)
- Sequence:
Ensembl Acc Id: ENSRNOP00000018259   ⟸   ENSRNOT00000018259
RefSeq Acc Id: XP_038957638   ⟸   XM_039101710
- Peptide Label: isoform X1
Protein Domains
DHFR

Protein Structures
Name Modeler Protein Id AA Range Protein Structure
AF-Q920D2-F1-model_v2 AlphaFold Q920D2 1-187 view protein structure

Transcriptome

eQTL   View at Phenogen
WGCNA   View at Phenogen
Tissue/Strain Expression   View at Phenogen

Promoters
RGD ID:13691058
Promoter ID:EPDNEW_R1582
Type:initiation region
Name:Dhfr_1
Description:dihydrofolate reductase
SO ACC ID:SO:0000170
Source:EPDNEW (Eukaryotic Promoter Database, http://epd.vital-it.ch/)
Experiment Methods:Single-end sequencing.
Position:
Rat AssemblyChrPosition (strand)Source
Rnor_6.0221,931,914 - 21,931,974EPDNEW

Additional Information

Database Acc Id Source(s)
AGR Gene RGD:2500 AgrOrtholog
BioCyc Gene G2FUF-54868 BioCyc
BioCyc Pathway 1CMET2-PWY [folate transformations III (E. coli)] BioCyc
  PWY66-385 [dTMP de novo biosynthesis (mitochondrial)] BioCyc
BioCyc Pathway Image 1CMET2-PWY BioCyc
  PWY66-385 BioCyc
Ensembl Genes ENSRNOG00000013521 Ensembl, UniProtKB/Swiss-Prot
  ENSRNOG00000082925 ENTREZGENE
Ensembl Transcript ENSRNOT00000018259.6 UniProtKB/Swiss-Prot
  ENSRNOT00000164530 ENTREZGENE
Gene3D-CATH 3.40.430.10 UniProtKB/Swiss-Prot
IMAGE_CLONE IMAGE:9022774 IMAGE-MGC_LOAD
InterPro DHFR UniProtKB/Swiss-Prot
  DHFR-like_dom_sf UniProtKB/Swiss-Prot
  DHFR_CS UniProtKB/Swiss-Prot
  DHFR_dom UniProtKB/Swiss-Prot
KEGG Report rno:24312 UniProtKB/Swiss-Prot
MGC_CLONE MGC:187341 IMAGE-MGC_LOAD
NCBI Gene 24312 ENTREZGENE
PANTHER DIHYDROFOLATE REDUCTASE UniProtKB/Swiss-Prot
  PTHR48069 UniProtKB/Swiss-Prot
Pfam DHFR_1 UniProtKB/Swiss-Prot
PharmGKB DHFR RGD
PhenoGen Dhfr PhenoGen
PRINTS DHFR UniProtKB/Swiss-Prot
PROSITE DHFR_1 UniProtKB/Swiss-Prot
  DHFR_2 UniProtKB/Swiss-Prot
RatGTEx ENSRNOG00000013521 RatGTEx
  ENSRNOG00000082925 RatGTEx
Superfamily-SCOP SSF53597 UniProtKB/Swiss-Prot
UniProt A0ABK0L5E9_RAT UniProtKB/TrEMBL
  A0ABK0L5R0_RAT UniProtKB/TrEMBL
  B0BMV8 ENTREZGENE, UniProtKB/TrEMBL
  DYR_RAT UniProtKB/Swiss-Prot, ENTREZGENE


Nomenclature History
Date Current Symbol Current Name Previous Symbol Previous Name Description Reference Status
2002-11-06 Dhfr  dihydrofolate reductase  Dhfr1  Dihydrofolate reductase 1 (active)  Symbol and Name updated 625702 APPROVED
2002-06-10 Dhfr1  Dihydrofolate reductase 1 (active)      Symbol and Name status set to approved 70586 APPROVED

RGD Curation Notes
Note Type Note Reference
gene_process important for reducing dihydrofolate to tetrahydrofolate for subsequent purine and pyrimidine biosynthesis 1298877