RGD Reference Report - Does neuroglobin protect neurons from ischemic insult? A quantitative investigation of neuroglobin expression following transient MCAo in spontaneously hypertensive rats.

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Does neuroglobin protect neurons from ischemic insult? A quantitative investigation of neuroglobin expression following transient MCAo in spontaneously hypertensive rats.
Authors:	Hundahl, C Kelsen, J Kjaer, K Ronn, LC Weber, RE Geuens, E Hay-Schmidt, A Nyengaard, JR
Citation:	Hundahl C, etal., Brain Res. 2006 Apr 26;1085(1):19-27. Epub 2006 May 2.
RGD ID:	9743952
Pubmed:	PMID:16647691 (View Abstract at PubMed)
DOI:	DOI:10.1016/j.brainres.2006.02.040 (Journal Full-text)
Neuroglobin (NGB) is a recently characterized heme globin expressed primarily in retinal nerve cells and at very low levels in endocrine-active regions of vertebrate brains. When artificially over-expressed, NGB reduces the infarct size observed after transient Middle Cerebral Artery occlusion (tMCAo) in rats. This study addresses the post-ischemic NGB expression in vivo. Ten Spontaneously Hypertensive Rats (SHRs) were randomized to tMCAo (n = 6) or sham (n = 4), and euthanized 24 h later. NGB mRNA expression was determined by means of quantitative Reverse Transcription Polymerase Reaction (qRT-PCR). Thirteen animals subjected to either 90 min tMCAo (n = 7) or sham (n = 6) surgery, were euthanized 1 week after surgery. Post-ischemic expression of NGB and the neuronal marker NeuN was studied using free-floating immunohistochemistry. Design-based stereological quantification of NGB- and NeuN-positive cells in the striatum was performed using the optical fractionator. Significantly less NGB mRNA was expressed in the ischemic hemispheres of tMCAo animals after 24 h (P < or = 0.002). At the protein level, we found a significantly lower number of NGB- and NeuN-positive striatal neurons in tMCAo rats (P < or = 0.004). NGB expression was mainly confined to the hypothalamus and amygdala. Less than one out of every two thousand neurons expressed NGB in the striatum. In the ischemic territory we did not observe selective sparing of NGB expressing neurons. No significant change in the NGB/NeuN ratio was observed. Our data indicate that endogenous expressed NGB does not provide protection against ischemic injury induced by tMCAo in SHRs.

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Term	Qualifier	Evidence	With	Reference	Notes	Source	Original Reference(s)
transient cerebral ischemia		ISO	Ngb (Rattus norvegicus)	9743952; 9743952	associated with Hypertension more ...	RGD
transient cerebral ischemia		IEP		9743952	associated with Hypertension more ...	RGD

Objects Annotated

Genes (Rattus norvegicus)

Ngb (neuroglobin)

Genes (Mus musculus)

Ngb (neuroglobin)

Genes (Homo sapiens)

NGB (neuroglobin)

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Does neuroglobin protect neurons from ischemic insult? A quantitative investigation of neuroglobin expression following transient MCAo in spontaneously hypertensive rats.