RGD Reference Report - Transient GFER knockdown in vivo impairs liver regeneration after partial hepatectomy. - Rat Genome Database

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Transient GFER knockdown in vivo impairs liver regeneration after partial hepatectomy.

Authors: Francavilla, A  Pesetti, B  Barone, M  Morgano, A  Bovenga, F  Napoli, A  Resta, L  Russo, F  Linsalata, M  Minoia, M  Bianco, G  Tafaro, A  Polimeno, L 
Citation: Francavilla A, etal., Int J Biochem Cell Biol. 2014 Aug;53:343-51. doi: 10.1016/j.biocel.2014.05.029. Epub 2014 May 28.
RGD ID: 9685725
Pubmed: PMID:24880092   (View Abstract at PubMed)
DOI: DOI:10.1016/j.biocel.2014.05.029   (Journal Full-text)

BACKGROUND AND AIMS: Augmenter of Liver Regeneration is a protein encoded by the Growth Factor Erv1-Like gene. Its biological properties are crucial for cell survival since knock-out mice for Growth Factor Erv1-Like gene do not survive. In this study, we injected hepatotropic adenoviral particles harboring oligonucleotide sequences against Growth Factor Erv1-Like gene into 70% partially hepatectomized rats and studied the effect of gene silencing on the progression liver regeneration. METHODS: Partially hepatectomized rats were divided into three groups of animals and, before surgery, received either phosphate buffer saline, or adenoviral particles alone or adenoviral particles harboring the oligonucleotide silencing sequence. In each group, rats were sacrificed at 12, 24 and 48 h after surgery. Liver tissues were collected to analyze the expression of Augmenter of Liver Regeneration, Bax, Bcl-2 and activated Caspase-9 and -3, as well as hepatocyte proliferation and apoptosis, polyamines levels and histological and ultrastructural features. RESULTS: Growth Factor Erv1-Like gene silencing reduced the compensatory hepatocellular proliferation triggered by surgery through (i) the reduction of polyamines synthesis, hepatocyte proliferation and anti-apoptotic gene expression and (ii) the increase of pro-apoptotic gene expression and caspase activation. CONCLUSIONS: For the first time, using a technique of gene silencing in vivo, our results demonstrate that Growth Factor Erv1-Like gene knock-down, i.e., the lack of Augmenter of Liver Regeneration, modifies the expression of genes involved in cell apoptosis and inhibits early phase of DNA synthesis. As a consequence, a promotion of cell death and a reduction of cell proliferation occurs.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Liver Injury  ISOGfer (Rattus norvegicus)9685725; 9685725 RGD 
Liver Injury  IEP 9685725 RGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
liver regeneration  IMP 9685725 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Gfer  (growth factor, augmenter of liver regeneration)

Genes (Mus musculus)
Gfer  (growth factor, augmenter of liver regeneration)

Genes (Homo sapiens)
GFER  (growth factor, augmenter of liver regeneration)


Additional Information