RGD Reference Report - MiR-15b and miR-152 reduce glioma cell invasion and angiogenesis via NRP-2 and MMP-3. - Rat Genome Database

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MiR-15b and miR-152 reduce glioma cell invasion and angiogenesis via NRP-2 and MMP-3.

Authors: Zheng, X  Chopp, M  Lu, Y  Buller, B  Jiang, F 
Citation: Zheng X, etal., Cancer Lett. 2013 Feb 28;329(2):146-54. doi: 10.1016/j.canlet.2012.10.026. Epub 2012 Nov 7.
RGD ID: 8694107
Pubmed: PMID:23142217   (View Abstract at PubMed)
PMCID: PMC3553580   (View Article at PubMed Central)
DOI: DOI:10.1016/j.canlet.2012.10.026   (Journal Full-text)

We tested invasion and angiogenesis related mRNA expression and miRNA profiles of glioma. Genes with mRNA expression that changed significantly were selected to predict possible miRNAs that regulate mRNA expression, and were then matched with miRNA results. NRP-2 with the matching miRNA miR-15b, and MMP-3 with the matching miRNA miR-152 were selected for further study. Luciferase activity assay confirmed that miR-15b and miR-152 attenuate expression of NRP-2 and MMP-3 protein by binding to NRP-2 and MMP-3 transcript, respectively. In vitro invasion assay data showed that miR-15b and miR-152 significantly decreased 9L cell invasiveness. In vitro tube formation assay data showed that miR-15b reduced tube formation. A preliminary pathway study indicated that miR-15b and miR-152 deactivated the MEK-ERK pathway via NRP-2 and MMP-3 in 9L cells, respectively.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
positive regulation of cell migration  IMP 8694107 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Mmp3  (matrix metallopeptidase 3)


Additional Information