RGD Reference Report - A dynamin-3 spliced variant modulates the actin/cortactin-dependent morphogenesis of dendritic spines. - Rat Genome Database

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A dynamin-3 spliced variant modulates the actin/cortactin-dependent morphogenesis of dendritic spines.

Authors: Gray, NW  Kruchten, AE  Chen, J  McNiven, MA 
Citation: Gray NW, etal., J Cell Sci. 2005 Mar 15;118(Pt 6):1279-90. Epub 2005 Mar 1.
RGD ID: 8554738
Pubmed: PMID:15741233   (View Abstract at PubMed)
DOI: DOI:10.1242/jcs.01711   (Journal Full-text)

Immature dendrites extend many actin-rich filopodial structures that can be replaced by synapse-containing dendritic spines as the neuron matures. The large GTPase dynamin-3 (Dyn3) is a component of the postsynapse in hippocampal neurons but its function is undefined. Here, we demonstrate that a specific Dyn3 variant (Dyn3baa) promotes the formation of immature dendritic filopodia in cultured neurons. This effect is dependent upon Dyn3 GTPase activity and a direct interaction with the F-actin-binding protein cortactin. Consistent with these findings, Dyn3baa binds to cortactin with a 200% higher affinity than Dyn3aaa, a near identical isoform that does not induce dendritic filopodia when expressed in cultured neurons. Finally, levels of Dyn3baa-encoding mRNA are tightly regulated during neuronal maturation and are markedly upregulated during synaptogenesis. Together, these findings provide the first evidence that an enhanced interaction between a specific Dyn3 splice variant and cortactin modulate actin-membrane dynamics in developing neurons to regulate the morphogenesis of dendritic spines.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
filopodium assembly involved_inIDA 8554738PMID:15741233UniProt 
regulation of dendritic spine morphogenesis  IMP 8554738 RGD 
synapse assembly involved_inIDA 8554738PMID:15741233UniProt 

Molecular Function

Objects Annotated

Genes (Rattus norvegicus)
Cttn  (cortactin)
Dnm3  (dynamin 3)


Additional Information