RGD Reference Report - Cloning and characterization of the rat GALR1 galanin receptor from Rin14B insulinoma cells. - Rat Genome Database

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Cloning and characterization of the rat GALR1 galanin receptor from Rin14B insulinoma cells.

Authors: Parker, EM  Izzarelli, DG  Nowak, HP  Mahle, CD  Iben, LG  Wang, J  Goldstein, ME 
Citation: Parker EM, etal., Brain Res Mol Brain Res 1995 Dec 28;34(2):179-89.
RGD ID: 728875
Pubmed: PMID:8750821   (View Abstract at PubMed)

Galanin is a ubiquitous neuropeptide that regulates a wide array of physiological processes via interaction with specific G protein-coupled receptors. A rat galanin receptor cDNA was cloned from the Rin14B insulinoma cell line. The isolated cDNA encodes a 346 amino acid G protein-coupled receptor that is 92% identical to the recently reported human GALR1 galanin receptor. [125I]Galanin binds with high affinity to two receptor states in COS1 cell membranes containing the rat GALR1 receptor, consistent with coupling of the receptor to a G protein in these membranes. N-terminal galanin fragments and the putative galanin receptor antagonists galantide, C7, M35 and M40 bind with high affinity to the rat GALR1 receptor. In contrast, C-terminal galanin fragments do not bind to this receptor. Galanin inhibits basal and forskolin-stimulated cAMP formation in CHO cells expressing the rat GALR1 receptor via a pertussis toxin-sensitive G protein. The GALR1 receptor is expressed in rat spinal cord, small intestine, Rin14B insulinoma cells and several brain regions, particularly ventral hippocampus, amygdala, supraoptic nucleus, hypothalamus, thalamus, lateral parabrachial nucleus and locus coeruleus. Cloning of the rat GALR1 galanin receptor cDNA will permit many new experimental strategies to be applied to studies of the structure and function of galanin receptors.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
G protein-coupled receptor signaling pathway  IDA 728875 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
galanin receptor activity  IDA 728875 RGD 
neuropeptide binding  IDA 728875 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Galr1  (galanin receptor 1)


Additional Information