RGD Reference Report - Discrimination between signaling pathways in regulation of specific gene expression by insulin and growth hormone in hepatocytes. - Rat Genome Database

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Discrimination between signaling pathways in regulation of specific gene expression by insulin and growth hormone in hepatocytes.

Authors: Ribaux, P  Gjinovci, A  Sadowski, HB  Iynedjian, PB 
Citation: Ribaux P, etal., Endocrinology 2002 Oct;143(10):3766-72.
RGD ID: 625703
Pubmed: PMID:12239086   (View Abstract at PubMed)
DOI: DOI:10.1210/en.2002-220304   (Journal Full-text)

Insulin and GH can activate common signaling elements in many tissues and cell lines. We investigated the possibility of overlap in signaling pathways activated by insulin and GH in a key target cell, the hepatocyte. In primary cultures of rat hepatocytes, GH caused a dose- and time-dependent increase in tyrosine phosphorylation of signal transducer and activator of transcription 5. This was accompanied by the induction of the mRNA encoding suppressor of cytokine signaling 2. Neither of these effects took place in companion hepatocytes challenged with insulin. By contrast, insulin caused a rapid and sustained phosphorylation of protein kinase B, accompanied by a massive induction of the mRNA encoding glucokinase. GH had no detectable effect on phosphorylation of protein kinase B or level of glucokinase mRNA. Insulin also elicited brief hyperphosphorylation of ERK1 and 2, an effect not seen in GH-stimulated hepatocytes. Thus, there was a clear demarcation of signaling events triggered in hepatocytes by insulin and GH, and this was accompanied by hormone-specific responses with respect to the induction of gene expression. Additionally, the current results show that signal transducer and activator of transcription 5 activation is neither necessary nor sufficient for the insulin-dependent induction of hepatic glucokinase.

Objects referenced in this article
Gene Gh1 growth hormone 1 Rattus norvegicus
Gene Ins1 insulin 1 Rattus norvegicus

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