RGD Reference Report - The effect of aluminium on NTPDase and 5'-nucleotidase activities from rat synaptosomes and platelets. - Rat Genome Database

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The effect of aluminium on NTPDase and 5'-nucleotidase activities from rat synaptosomes and platelets.

Authors: Kaizer, RR  Maldonado, PA  Spanevello, RM  Correa, MC  Goncalves, JF  Becker, LV  Morsch, VM  Schetinger, MR 
Citation: Kaizer RR, etal., Int J Dev Neurosci. 2007 Oct;25(6):381-6. Epub 2007 Jul 10.
RGD ID: 5134345
Pubmed: PMID:17686601   (View Abstract at PubMed)
DOI: DOI:10.1016/j.ijdevneu.2007.06.002   (Journal Full-text)

Aluminium (Al), a neurotoxic compound, has been investigated in a large number of studies both in vivo and in vitro. In this study, we investigated the effect in vivo of long-term exposure to Al on NTPDase (nucleoside triphosphate diphosphohydrolase) and 5'-nucleotidase activities in the synaptosomes (obtained from the cerebral cortex and hippocampus) and platelets of rats. Here, we investigated a possible role of platelets as peripheral markers in rats. Rats were loaded by gavage with AlCl(3) 50 mg/(kg day), 5 days per week, totalizing 60 administrations. The animals were divided into four groups: (1) control (C), (2) 50 mg/kg of citrate solution (Ci), (3) 50 mg/kg of Al plus citrate (Al+Ci) solution and (4) 50 mg/kg of Al (Al). ATP hydrolysis was increased in the synaptosomes from the cerebral cortex by 42.9% for Al+Ci and 39.39% for Al, when compared to their respective control (p<0.05). ADP hydrolysis was increased by 13.15% for both Al and Al+Ci, and AMP hydrolysis increased by 32.7% for Al and 27.25% for Al+Ci (p<0.05). In hippocampal synaptosomes, the hydrolysis of ATP, ADP and AMP, was increased by 58.5%, 28.5% and 25.92%, respectively, for Al (p<0.05) and 36.7%, 22.5% and 37.64% for Al+Ci, both when compared to their respective controls. ATP, ADP and AMP hydrolysis, in platelets, was increased by 172.3%, 188.52% and 92.1%, respectively in Al+Ci, and 317.9%, 342.8% and 177.9%, respectively, for Al, when compared to their respective controls (p<0.05). Together, these results indicate that Al increases NTPDase and 5'-nucleotidase activities, in synaptosomal fractions and platelets. Thus, we suggest that platelets could be sensitive peripheral markers of Al toxicity of the central nervous system.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
response to aluminum ion  IEP 5134345 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Nt5e  (5' nucleotidase, ecto)


Additional Information