RGD Reference Report - Transformation, growth rate, and the heme biosynthetic pathway in V-abl-transfected fibroblasts. - Rat Genome Database

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Transformation, growth rate, and the heme biosynthetic pathway in V-abl-transfected fibroblasts.

Authors: Mamet, R  Teitz, Y  Schoenfeld, N 
Citation: Mamet R, etal., Biochem Med Metab Biol. 1994 Jun;52(1):53-7.
RGD ID: 4144799
Pubmed: PMID:7917467   (View Abstract at PubMed)

The relationship between growth rate and various parameters of the heme biosynthetic pathway was studied in two cell lines of rat fibroblasts (REabl-1 and REabl-3) transfected with v-abl oncogene, coded by the Abelson murine leukemia virus, and subjected to glucocorticoid dependent transformation. In the REabl-1 cell line, whose growth rate was only slightly affected by dexamethasone (DX), almost no change was noticed either in heme content or in the enzymatic activities of aminolevulinate synthase (ALAS), porphobilinogen deaminase (PBGD), and ferrochelatase (FC) in the presence of various concentrations of DX. In the REabl-3 cell line, exhibiting a growth rate highly sensitive to DX, a significant reduction in intracellular heme concomitantly with decreases in ALAS and FC activities and a threefold increase in PBGD were noted. The fact that incubation with 10(-5)M hemin did not result in a decrease in ALAS activity raised the possibility that REabl cells lack a negative feedback control mechanism. The relationships between transformation, growth rate, and heme biosynthetic pathway are discussed.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cellular response to dexamethasone stimulus  IEP 4144799; 4144799 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Fech  (ferrochelatase)
Hmbs  (hydroxymethylbilane synthase)


Additional Information