RGD Reference Report - Immunohistochemistry of cerebellar seizures: mossy fiber afferents play an important role in seizure spread and initiation in the rat. - Rat Genome Database

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Immunohistochemistry of cerebellar seizures: mossy fiber afferents play an important role in seizure spread and initiation in the rat.

Authors: Tóth, Zoltán  Molnár, Gergely  Mihály, András  Krisztin-Péva, Beáta  Morvai, Marietta  Kopniczky, Zsolt 
Citation: Tóth Z, etal., Acta Histochem. 2015 Jan;117(1):47-55. doi: 10.1016/j.acthis.2014.11.002. Epub 2014 Nov 22.
RGD ID: 405650614
Pubmed: PMID:25466986   (View Abstract at PubMed)
DOI: DOI:10.1016/j.acthis.2014.11.002   (Journal Full-text)

Clinical reports suggest the participation of the cerebellum in epilepsy. Mossy fibers are the main excitatory afferents of the cerebellar cortex; most of them use glutamate and strongly excite granule cells through NMDA and AMPA receptors. The role of the ponto-cerebellar mossy fibers in cerebellar neuronal hyperactivity was investigated in the present study in experimental adult Wistar rats. We detected neuronal hyperactivity through the expression of the glutamate-induced c-fos protein, by means of immunohistochemistry and immunoblotting in the vermis and in the hemispheres. Generalized seizures were induced by means of intraperitoneal 4-aminopyridine injections. Following the 4-aminopyridine seizures, the c-fos expression of cerebellar granule cells was significantly elevated at 1.5h in every lobule. Maximum c-fos expression was seen at 3h. The role of the ponto-cerebellar mossy fiber afferents in the induction of c-fos expression was examined after the transection of the middle cerebellar peduncle on the left side. Immunohistochemical analysis 14 days after the surgery revealed that the synapsin I immunoreactivity was significantly reduced in the cerebellar cortex on the operated side, compared to the sham-operated controls and to the non-operated cerebellar hemisphere of the operated animals, indicating the degeneration of mossy fiber terminals. Transection of the middle cerebellar peduncle suppressed cerebellar c-fos expression in the vermis and in the hemispheres significantly. These findings suggest the strong involvement of the middle cerebellar peduncle and the ponto-cerebellar mossy fibers in the pathophysiology of cerebellar epilepsy.



RGD Manual Disease Annotations    Click to see Annotation Detail View
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
FOSHumanepilepsy  ISOFos (Rattus norvegicus)protein:increased expression:cerebellumRGD 
FosRatepilepsy  IEP protein:increased expression:cerebellumRGD 
FosMouseepilepsy  ISOFos (Rattus norvegicus)protein:increased expression:cerebellumRGD 

Objects Annotated

Genes (Rattus norvegicus)
Fos  (Fos proto-oncogene, AP-1 transcription factor subunit)

Genes (Mus musculus)
Fos  (FBJ osteosarcoma oncogene)

Genes (Homo sapiens)
FOS  (Fos proto-oncogene, AP-1 transcription factor subunit)


Additional Information