RGD Reference Report - Protein tyrosine phosphatase receptor type delta (PTPRD) gene in an animal model of restless legs syndrome. - Rat Genome Database

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Protein tyrosine phosphatase receptor type delta (PTPRD) gene in an animal model of restless legs syndrome.

Authors: Morais, Milca Abda  Franco, Beatriz Silva  Holanda, Alessandro Spencer Souza  de Paula Simino, Laís Angélica  Veras, Alana Carolina Costa  Torsoni, Marcio Alberto  Manconi, Mauro  Torsoni, Adriana Souza  Esteves, Andrea Maculano 
Citation: Morais MA, etal., J Sleep Res. 2023 Apr;32(2):e13716. doi: 10.1111/jsr.13716. Epub 2022 Sep 2.
RGD ID: 401976457
Pubmed: PMID:36053904   (View Abstract at PubMed)
DOI: DOI:10.1111/jsr.13716   (Journal Full-text)

The pathophysiology of the restless legs syndrome (RLS) is related to dopaminergic dysfunction, reduced iron and variations in gene expression, such as the protein tyrosine phosphatase receptor type delta gene (PTPRD). Animal models could be key to achieving a mechanistic understanding of RLS and to facilitate efficient platforms for evaluating new therapeutics. Thus, the aim of this study was to evaluate the expression of PTPRD, of genes and proteins associated with RLS, the sleep patterns and the cardiovascular parameters in an animal model of RLS (spontaneously hypertensive rat [SHR]). Rats were divided into two groups: (i) Wistar-Kyoto and (ii) SHR. Cardiovascular parameters were assessed by tail plethysmography. Polysomnography was used to analyse the sleep pattern (24 h). For the PTPRD analyses, quantitative polymerase chain reaction (qPCR) and indirect enzyme-linked immunosorbent assay (ELISA) techniques were used. To evaluate the tyrosine hydroxylase enzyme, dopamine transporter (DAT) and type 2 dopaminergic receptor, qPCR and Western Blotting techniques were used. For the quantification of iron, ferritin and transferrin, the ELISA method was used. SHRs had higher blood pressure, alterations in sleep pattern, lower expression of protein content of PTPRD, lower expression of DAT, and lower serum concentrations of ferritin. These data suggest that the behavioural, physiological, and molecular changes observed in SHRs provide a useful animal model of RLS, reinforcing the importance of this strain as an animal model of this sleep disorder.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
restless legs syndrome  ISOPtprd (Rattus norvegicus)401976457; 401976457protein:decreased expression:striatum (rat)RGD 
restless legs syndrome  IEP 401976457protein:decreased expression:striatum (rat)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Ptprd  (protein tyrosine phosphatase, receptor type, D)

Genes (Mus musculus)
Ptprd  (protein tyrosine phosphatase receptor type D)

Genes (Homo sapiens)
PTPRD  (protein tyrosine phosphatase receptor type D)


Additional Information