RGD Reference Report - Water extract of Cnidii Rhizoma suppresses RANKL-induced osteoclastogenesis in RAW 264.7 cell by inhibiting NFATc1/c-Fos signaling and prevents ovariectomized bone loss in SD-rat. - Rat Genome Database

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Water extract of Cnidii Rhizoma suppresses RANKL-induced osteoclastogenesis in RAW 264.7 cell by inhibiting NFATc1/c-Fos signaling and prevents ovariectomized bone loss in SD-rat.

Authors: Lee, Ka-Yeon  Kim, Jae-Hyun  Kim, Eun-Young  Yeom, Mijung  Jung, Hyuk-Sang  Sohn, Youngjoo 
Citation: Lee KY, etal., BMC Complement Altern Med. 2019 Aug 9;19(1):207. doi: 10.1186/s12906-019-2611-8.
RGD ID: 329328926
Pubmed: PMID:31399090   (View Abstract at PubMed)
PMCID: PMC6688344   (View Article at PubMed Central)
DOI: DOI:10.1186/s12906-019-2611-8   (Journal Full-text)


BACKGROUND: Cnidii Rhizoma is the dried root stem of Cnidium officinale Makino. Cnidii Rhizoma (CR) has been used to treat menstrual irregularity, menstrual pain, and menopause in Korea. However, the effects and mechanisms of CR on RANKL-induced osteoclastogenesis pathway remain to be elucidated. In this study, we investigated the effects of CR on the inhibition of bone resorption of osteoclast and its mechanism RANK signaling pathway.
METHODS: The anti-osteoclastogenesis of water extract of CR was measured using RAW 264.7 cell. Tartrate-resistant acid phosphatase (TRAP) assay, pit assay, reverse transcription polymerase chain reaction (RT-PCR) and western blot were performed. Moreover, the effects of CR were determined with an in vivo model using ovariectomized (OVX) rats.
RESULTS: CR extract suppressed osteoclastogenesis, its activity and bone resorption activity through decreasing gene of osteoclast-related such as nuclear factor of activated T-cells, cytoplasmic 1 (NFATc1), c-Fos, etc. Moreover, CR extract prevented the bone loss in OVX rats.
CONCLUSION: These results show that CR has a positive effect on menopausal osteoporosis by suppressing osteoclastogenesis.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Postmenopausal Osteoporosis treatmentISONfatc1 (Rattus norvegicus)329328926; 329328926 RGD 
Postmenopausal Osteoporosis treatmentIEP 329328926 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Nfatc1  (nuclear factor of activated T-cells 1)

Genes (Mus musculus)
Nfatc1  (nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 1)

Genes (Homo sapiens)
NFATC1  (nuclear factor of activated T cells 1)


Additional Information