RGD Reference Report - Externalization of endogenous annexin A5 participates in apoptosis of rat cardiomyocytes. - Rat Genome Database

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Externalization of endogenous annexin A5 participates in apoptosis of rat cardiomyocytes.

Authors: Monceau, V  Belikova, Y  Kratassiouk, G  Charue, D  Camors, E  Communal, C  Trouve, P  Russo-Marie, F  Charlemagne, D 
Citation: Monceau V, etal., Cardiovasc Res. 2004 Dec 1;64(3):496-506.
RGD ID: 2317544
Pubmed: PMID:15537503   (View Abstract at PubMed)
DOI: DOI:10.1016/j.cardiores.2004.08.003   (Journal Full-text)

OBJECTIVE: Annexins are Ca(2+)-dependent phospholipid binding proteins. Externalized annexin A5 has been recently suggested to have a proapoptotic effect. Our aim was to determine whether annexin A5, which is intracellular in cardiomyocytes, could be translocated and/or externalized and play a role during the apoptotic process. METHODS: Apoptosis was induced in rat cardiomyocytes by continuous incubation with staurosporine or 30 min treatment with H(2)O(2) and was measured by phosphatidylserine (PS) externalization, TUNEL staining and DNA ladder. Immunofluorescence labeling of annexin A5 was performed on permeabilized or nonpermeabilized cardiomyocytes. RESULTS: Staurosporine or H(2)O(2) treatment of neonatal cardiomyocytes resulted in significant increases of apoptosis at 24 h, but H(2)O(2) treatment led to a faster and higher PS externalization than that observed with ST. In both neonatal and adult cardiomyocytes, annexin A5 was intracellular in control conditions but was found at the external face of sarcolemma during apoptosis. Furthermore, neonatal cardiomyocytes with externalized annexin A5 have apoptotic characteristics and their number increased with time. Interestingly, immediately after H(2)O(2) induction, the number of annexin A5-positive cells was higher than that of PS-positive cells (p

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
positive regulation of apoptotic process  IDA 2317544 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Anxa5  (annexin A5)


Additional Information