RGD Reference Report - Role of nonreceptor protein tyrosine kinases during phospholipase C-gamma 1-related uterine contractions in the rat. - Rat Genome Database

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Role of nonreceptor protein tyrosine kinases during phospholipase C-gamma 1-related uterine contractions in the rat.

Authors: Phillippe, M  Sweet, LM  Bradley, DF  Engle, D 
Citation: Phillippe M, etal., Reprod Sci. 2009 Mar;16(3):265-73.
RGD ID: 2316554
Pubmed: PMID:19208792   (View Abstract at PubMed)
PMCID: PMC3089769   (View Article at PubMed Central)
DOI: DOI:10.1177/1933719108327598   (Journal Full-text)

Activated phospholipase C1, produced in response to tyrosine phosphorylation, appears to play an important role during uterine contractions. These studies sought to determine which non-receptor protein tyrosine kinases are involved in the activation of phospholipase C1 in rat uterine tissue. In vitro contraction studies were performed utilizing isoform specific protein tyrosine kinase inhibitors. Western blots were performed utilizing antibodies to phosphotyrosine-phospholipase C1, total phospholipase C1, c-Src kinase and Lck kinase. Spontaneous, stretch-stimulated, and bpV(phen) (tyrosine phosphatase inhibitor) enhanced uterine contractions were significantly suppressed in response to Damnacanthal (Lck kinase inhibitor) and PP1 (c-Src kinase inhibitor). Damnacanthal and PP1 also significantly suppressed bpV(phen)-enhanced tyrosine phosphorylation of phospholipase C1. Western blots confirmed expression of Lck kinase and c-Src kinase in uterine tissue. In conclusion, the Lck and c-Src kinases appear to play an important role in regulating tyrosine phosphorylation of phospholipase C1 and contractile activity in the rat uterus.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
LckRatpositive regulation of uterine smooth muscle contraction  IMP  RGD 
LckRatresponse to mechanical stimulus  IMP  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Lck  (LCK proto-oncogene, Src family tyrosine kinase)


Additional Information