RGD Reference Report - Identification of a rabphilin-3A-interacting protein as GTP cyclohydrolase I in PC12 cells. - Rat Genome Database

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Identification of a rabphilin-3A-interacting protein as GTP cyclohydrolase I in PC12 cells.

Authors: Imazumi, K  Sasaki, T  Takahashi, K  Takai, Y 
Citation: Imazumi K, etal., Biochem Biophys Res Commun. 1994 Dec 15;205(2):1409-16.
RGD ID: 2314916
Pubmed: PMID:7802677   (View Abstract at PubMed)
DOI: DOI:10.1006/bbrc.1994.2822   (Journal Full-text)

Rabphilin-3A is a putative target protein for Rab3A small GTP-binding protein which is implicated in neurotransmitter release. To identify a Rabphilin-3A-interacting protein, proteins were immunoprecipitated by an anti-Rabphilin-3A polyclonal antibody from the lysate of PC12 cells and subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis followed by protein staining. Several proteins were coimmunoprecipitated with Rabphilin-3A and one of these proteins with a M(r) of about 30 KDa was phosphorylated in intact PC12 cells stimulated by high KCl. The amino acid sequence analysis of this 30 KDa protein revealed that it is GTP cyclohydrolase I.

Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
protein binding  IPIRph3a (Rattus norvegicus)2314916 RGD 
protein binding  IPIGch1 (Rattus norvegicus)2314916 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Gch1  (GTP cyclohydrolase 1)
Rph3a  (rabphilin 3A)


Additional Information