RGD Reference Report - FGF-2-responsive neural stem cell proliferation requires CCg, a novel autocrine/paracrine cofactor. - Rat Genome Database

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FGF-2-responsive neural stem cell proliferation requires CCg, a novel autocrine/paracrine cofactor.

Authors: Taupin, P  Ray, J  Fischer, WH  Suhr, ST  Hakansson, K  Grubb, A  Gage, FH 
Citation: Taupin P, etal., Neuron. 2000 Nov;28(2):385-97.
RGD ID: 2306506
Pubmed: PMID:11144350   (View Abstract at PubMed)

We have purified and characterized a factor, from the conditioned medium of neural stem cell cultures, which is required for fibroblast growth factor 2's (FGF-2) mitogenic activity on neural stem cells. This autocrine/paracrine cofactor is a glycosylated form of cystatin C (CCg), whose N-glycosylation is required for its activity. We further demonstrated that, both in vitro and in vivo, neural stem cells undergoing cell division are immunopositive for cystatin C. Finally, we showed in vivo functional activity of CCg by demonstrating that the combined delivery of FGF-2 and CCg to the adult dentate gyrus stimulated neurogenesis. We propose that the process of neurogenesis is controlled by the cooperation between trophic factors and autocrine/paracrine cofactors, of which CCg is a prototype.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
positive regulation of cell population proliferation  IDA 2306506Glycosylated cystatin C and rat hippocampal progenitorsRGD 

Objects Annotated

Genes (Rattus norvegicus)
Cst3  (cystatin C)


Additional Information