RGD Reference Report - GAIP, GIPC and Galphai3 are concentrated in endocytic compartments of proximal tubule cells: putative role in regulating megalin's function. - Rat Genome Database

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GAIP, GIPC and Galphai3 are concentrated in endocytic compartments of proximal tubule cells: putative role in regulating megalin's function.

Authors: Lou, X  McQuistan, T  Orlando, RA  Farquhar, MG 
Citation: Lou X, etal., J Am Soc Nephrol. 2002 Apr;13(4):918-27.
RGD ID: 2303813
Pubmed: PMID:11912251   (View Abstract at PubMed)

Megalin is the most abundant endocytic receptor in the proximal tubule epithelium (PTE), where it is concentrated in clathrin-coated pits (CCPs) and vesicles in the brush border region. The heterotrimeric G protein alpha subunit, Galphai3, has also been localized to the brush border region of PTE. By immunofluorescence GIPC and GAIP, components of G protein-mediated signaling pathways, are also concentrated in the brush border region of PTE and are present in megalin-expressing cell lines. By cell fractionation, these signaling molecules cosediment with megalin in brush border and microvillar fractions. GAIP is found by immunoelectron microscopy in CCPs, and GIPC is found in CCPs and apical tubules of endocytic compartments in the renal brush border. In precipitation assays, GST-GIPC specifically binds megalin. The concentration of Galphai3, GIPC, and GAIP with megalin in endocytic compartments of the proximal tubule, where extensive endocytosis occurs, and the interaction between GIPC and the cytoplasmic tail of megalin suggest a model whereby G protein-mediated signaling may regulate megalin's endocytic function and/or trafficking.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
brush border  IDA 2303813; 2303813; 2303813 RGD 
clathrin-coated vesicle  IDA 2303813 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Gipc1  (GIPC PDZ domain containing family, member 1)
Lrp2  (LDL receptor related protein 2)
Rgs19  (regulator of G-protein signaling 19)


Additional Information