RGD Reference Report - Long-term feeding of various fat diets modulates azoxymethane-induced colon carcinogenesis through Wnt/beta-catenin signaling in rats. - Rat Genome Database

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Long-term feeding of various fat diets modulates azoxymethane-induced colon carcinogenesis through Wnt/beta-catenin signaling in rats.

Authors: Fujise, T  Iwakiri, R  Kakimoto, T  Shiraishi, R  Sakata, Y  Wu, B  Tsunada, S  Ootani, A  Fujimoto, K 
Citation: Fujise T, etal., Am J Physiol Gastrointest Liver Physiol. 2007 Apr;292(4):G1150-6. Epub 2006 Dec 28.
RGD ID: 2298807
Pubmed: PMID:17194898   (View Abstract at PubMed)
DOI: DOI:10.1152/ajpgi.00269.2006   (Journal Full-text)

The Wnt signaling pathway plays an essential role in carcinogenesis, and the amount of fat intake and composition of dietary fatty acids are crucial factors for colon carcinogenesis. We investigated whether various dietary fats affected the Wnt signaling pathway of colon tumorigenesis in azoxymethane (AOM)-treated rats. Male Sprague-Dawley rats were given intraperitoneal injections of AOM and supplemented with 10% corn, olive, beef, and fish oil for 44 wk. Aberrant crypt foci (ACF) and tumors were examined at 12 and 44 wk. Normal appearing colon mucosal proliferation and apoptosis were evaluated by 5-bromo-2'-deoxyuridine (BrdU) incorporation and percentages of fragmented DNA, respectively. Expressions of beta-catenin, cyclin D(1), Wnt2, Wnt3, and Wnt5a of normal appearing colon mucosa were analyzed by Western blot analysis. Long-term dietary corn oil and beef tallow increased ACF, tumor incidence, and tumor numbers in AOM-treated rats. In contrast, both olive and fish oil inhibited them. Dietary corn oil and beef tallow increased BrdU incorporation and the expression of cytosolic beta-catenin and cyclin D(1) and decreased apoptosis in the colon mucosa. Expressions of Wnt2 and Wnt3 in rats fed with beef tallow and Wnt5a in rats fed with corn oil increased with or without AOM-treatment. BrdU-incorporated cells were often observed at the tops of crypts in rats fed with beef tallow, whereas this was not observed in rats fed with the other diet. Long-term high intake of corn oil and beef tallow enhanced cell proliferation through Wnt signaling and modulated the distribution of proliferating cells, which might contribute to promoting effects in colon tumorigenesis.



Objects referenced in this article
Gene Wnt2 Wnt family member 2 Rattus norvegicus
Gene Wnt3 Wnt family member 3 Rattus norvegicus
Gene Wnt5a Wnt family member 5A Rattus norvegicus

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