RGD Reference Report - Telomerase RNA as a detection marker in the serum of breast cancer patients. - Rat Genome Database

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Telomerase RNA as a detection marker in the serum of breast cancer patients.

Authors: Chen, XQ  Bonnefoi, H  Pelte, MF  Lyautey, J  Lederrey, C  Movarekhi, S  Schaeffer, P  Mulcahy, HE  Meyer, P  Stroun, M  Anker, P 
Citation: Chen XQ, etal., Clin Cancer Res. 2000 Oct;6(10):3823-6.
RGD ID: 2291973
Pubmed: PMID:11051224   (View Abstract at PubMed)

Tumor-derived circulating DNA has been found in the plasma of cancer patients. Alterations include decreased strand stability, mutations of oncogenes or of tumor suppressor genes, microsatellite alterations, and hypermethylation of several genes. RNA has also been found circulating in the plasma of normal subjects and cancer patients. Tyrosinase mRNA has been extracted from the serum of melanoma patients and subjected to RT-PCR. Moreover, the presence of cell-free EBV-associated RNA has been reported in the plasma of patients with nasopharyngeal carcinoma. Human telomerase comprises two RNA subunits, telomerase RNA template (hTR) and its catalytic component, telomerase reverse transcriptase protein (hTERT). Expression of these subunits correlates with telomerase activity. Using RT-PCR, we investigated whether these RNA subunits were present in the serum of 18 patients with breast cancer, 2 patients with benign breast disease, and 21 normal subjects. The presence of amplifiable RNA was confirmed in all tissue and serum samples using RT-PCR of glyceraldehyde-3-phosphate dehydrogenase RNA. hTR was found in 17 of 18 tumors (94%) and 5 of 18 serum samples (28%). hTERT was also detected in 17 of 18 tumors (94%) and in 4 of 16 available serum samples (25%). hTR and hTERT were undetectable in tissues and sera taken from 2 patients with benign disease and in the sera of 21 normal subjects. We conclude that RNA is detectable in the serum of breast cancer patients and that tumor-derived mRNA can be extracted and amplified using RT-PCR, even in patients with localized disease. This may have implications for cancer diagnosis and follow-up in the future.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
breast cancer  IEP 2291973RNA:increased expression:serumRGD 
breast cancer  ISOTERC (Homo sapiens)2291973; 2291973RNA:increased expression:serumRGD 

Objects Annotated

Genes (Rattus norvegicus)
Terc  (telomerase RNA component)

Genes (Mus musculus)
Terc  (telomerase RNA component)

Genes (Homo sapiens)
TERC  (telomerase RNA component)


Additional Information