RGD Reference Report - The expression and function of Toll-like receptors 3 and 9 in human colon carcinoma. - Rat Genome Database

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The expression and function of Toll-like receptors 3 and 9 in human colon carcinoma.

Authors: Nojiri, Keiichiro  Sugimoto, Kazushi  Shiraki, Katsuya  Tameda, Masahiko  Inagaki, Yuuji  Kusagawa, Satoko  Ogura, Suguru  Tanaka, Junichiro  Yoneda, Misao  Yamamoto, Norihiko  Okano, Hiroshi  Takei, Yoshiyuki  Ito, Masaaki  Kasai, Chika  Inoue, Hidekazu  Takase, Koujiro 
Citation: Nojiri K, etal., Oncol Rep. 2013 May;29(5):1737-43. doi: 10.3892/or.2013.2322. Epub 2013 Mar 4.
RGD ID: 21079438
Pubmed: PMID:23467704   (View Abstract at PubMed)
DOI: DOI:10.3892/or.2013.2322   (Journal Full-text)

Toll-like receptors (TLRs) are pattern-recognition receptors that are important in immune signaling. TLR recognition of various viral components including double-stranded RNA (TLR3) and unmethylated CpG-DNA (TLR9) plays a crucial role in cell survival. However, TLR expression and function in colon carcinoma cells are not well clarified. We investigated the expression of TLR3 and TLR9 in colon carcinoma cells using immunohistochemical methods. The function of TLR3 and TLR9 signaling in carcinoma cell lines was studied by direct cell stimulation with, or by cell transfection of, polyinosinic-polycytidylic acid (Poly I:C), a synthetic form of dsRNA, and by cell stimulation with CpG-oligodeoxynucleotides (ODNs), respectively. Positive TLR3 and TLR9 immunohistochemical staining was observed in 91 and 86% of human hepatocellular carcinoma (HCC) tissues, respectively. Cell surface stimulation of TLR3 with Poly I:C did not affect cell viability but it did activate NF-κB activity. By contrast, stimulation of intracellular TLRs with transfected Poly I:C significantly induced apoptosis. Cell surface stimulation of TLR9 with CpG-ODNs promoted cell proliferation, and, furthermore, these CpG-ODN TLR9 agonists reduced the cytotoxicity of the anticancer drug adriamycin. Cell surface expression of TLR3 and TLR9 in colon carcinoma cells plays an important role in cell survival. In addition, the proapoptotic activity of intracellularly expressed TLR3 may provide the possibility of using TLR3 agonists as novel clinical cytotoxic agents against colon carcinoma cells.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
colon carcinoma  IEP 21079438protein:increased expression:colonRGD 
colon carcinoma  ISOTLR3 (Homo sapiens)21079438; 21079438protein:increased expression:colonRGD 

Objects Annotated

Genes (Rattus norvegicus)
Tlr3  (toll-like receptor 3)

Genes (Mus musculus)
Tlr3  (toll-like receptor 3)

Genes (Homo sapiens)
TLR3  (toll like receptor 3)


Additional Information