RGD Reference Report - Altered expression of sodium transporters in ischemic penumbra after focal cerebral ischemia in rats. - Rat Genome Database

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Altered expression of sodium transporters in ischemic penumbra after focal cerebral ischemia in rats.

Authors: Jung, YW  Choi, IJ  Kwon, TH 
Citation: Jung YW, etal., Neurosci Res. 2007 Oct;59(2):152-9. Epub 2007 Jun 26.
RGD ID: 1642717
Pubmed: PMID:17662498   (View Abstract at PubMed)
DOI: DOI:10.1016/j.neures.2007.06.1470   (Journal Full-text)

This study was aimed to examine whether the changes of protein expression of sodium transporters in the ischemic penumbra are associated with the pathogenesis of ischemia-induced brain edema and/or brain cell injury. An experimental model of cerebral ischemia was made by permanent middle cerebral artery occlusion (pMCAO) in rats and the changes of protein expression of sodium transporters in the ischemic penumbra were examined by immunoblotting. Extensive infarction was observed in the frontal and parietal cortical and subcortical areas at 3 and 6h after pMCAO. Immunoblotting analyses revealed significantly increased expressions of electrogenic NBC (241+/-11% at 3h and 154+/-9% at 6h, P<0.05) and NHE1 (144+/-3% at 3h and 170+/-9% at 6h, P<0.05), compared with sham-operated controls. In contrast, Na-K-ATPase expression (78+/-6% at 3h and 85+/-3% at 6h, P<0.05) was significantly decreased. The expression of NCX1 was unchanged at 3h, but was significantly increased at 6h (141+/-3%, P<0.05). In addition, the expressions of neuronal (NeuN) and astroglial cell (GFAP) proteins were decreased, whereas the expression of oligodendrocyte protein (CNPase) was unchanged. Taken together, the selectively increased expressions of NHE1, electrogenic NBC, and NCX1 and decreased expression of Na-K-ATPase in the ischemic penumbra are likely to contribute to the secondary brain cell damages presumably through intracellular Na(+) accumulation, cell swelling, and intracellular Ca(2+) overload.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
brain ischemia  ISOSlc8a1 (Rattus norvegicus)1642717; 1642717protein:increased expression:brainRGD 
brain ischemia  IEP 1642717protein:increased expression:brainRGD 

Objects Annotated

Genes (Rattus norvegicus)
Slc8a1  (solute carrier family 8 member A1)

Genes (Mus musculus)
Slc8a1  (solute carrier family 8 (sodium/calcium exchanger), member 1)

Genes (Homo sapiens)
SLC8A1  (solute carrier family 8 member A1)


Additional Information