RGD Reference Report - Ethanol increases apolipoprotein B mRNA editing in rat primary hepatocytes and McArdle cells. - Rat Genome Database

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Ethanol increases apolipoprotein B mRNA editing in rat primary hepatocytes and McArdle cells.

Authors: Van Mater, D  Sowden, MP  Cianci, J  Sparks, JD  Sparks, CE  Ballatori, N  Smith, HC 
Citation: Van Mater D, etal., Biochem Biophys Res Commun. 1998 Nov 18;252(2):334-9.
RGD ID: 1626287
Pubmed: PMID:9826530   (View Abstract at PubMed)
DOI: DOI:10.1006/bbrc.1998.9647   (Journal Full-text)

Apolipoprotein B (apoB) mRNA editing involves a site-specific cytidine to uridine transition catalyzed by the cytidine deaminase, APOBEC-1, in the context of and regulated by a multi-protein-containing editosome. ApoB mRNA editing in vivo is subject to tissue specific, developmental and metabolic regulation. We demonstrate for the first time that the amount of edited apoB mRNA in rat primary hepatocytes is markedly increased subsequent to transient treatment with ethanol in vitro. The apparent change in editing efficiency was dose-dependent (from 0.1%-2.4% initial ethanol dose) and occurred with rapid onset. The proportion of edited apoB mRNA was also markedly enhanced in a rat hepatoma cell line, McArdle RH7777 cells and in a stable McArdle cell line over-expressing APOBEC-1 by transient treatment with 2.5 % ethanol. In contrast, the apoB mRNA editing in a human hepatoma cell line, HepG2 cells and a stable HepG2 cell line over-expressing APOBEC-1 did not respond to ethanol treatment. The data support the possibility that editing activity is ethanol-responsive but suggest that this change is cell type-specific.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Apobec1Ratresponse to ethanol  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Apobec1  (apolipoprotein B mRNA editing enzyme catalytic subunit 1)


Additional Information