RGD Reference Report - Calpain facilitates actin reorganization during glucose-stimulated insulin secretion. - Rat Genome Database

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Calpain facilitates actin reorganization during glucose-stimulated insulin secretion.

Authors: Turner, MD  Fulcher, FK  Jones, CV  Smith, BT  Aganna, E  Partridge, CJ  Hitman, GA  Clark, A  Patel, YM 
Citation: Turner MD, etal., Biochem Biophys Res Commun. 2007 Jan 19;352(3):650-5. Epub 2006 Nov 29.
RGD ID: 1625052
Pubmed: PMID:17150188   (View Abstract at PubMed)
DOI: DOI:10.1016/j.bbrc.2006.11.077   (Journal Full-text)

Calpain-10 (CAPN10) has been identified as a diabetes susceptibility gene. Previous studies have shown that alterations in calpain activity alter both glucose uptake and insulin secretion. In this report, we investigated the role of calpain activity in the actin reorganization required for glucose-stimulated insulin secretion. In pancreatic INS-1 cells, acute exposure to a high glucose environment stimulated CAPN10 gene expression with a concomitant increase in calpain activity. However, high glucose did not significantly alter expression of the two major ubiquitously expressed calpain family members, CAPN1 and CAPN2. Furthermore, glucose stimulation resulted in the reorganization of actin and inhibition of calpain activity impaired this reorganization in INS-1 cells. Finally, we identified a 54 kDa isoform as the major CAPN10 isoform that associates with the actin cytoskeleton. Based on our findings, we propose that calpain plays a role in facilitating the actin reorganization required for glucose-stimulated insulin secretion in INS-1 cells.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
response to glucose  IEP 1625052 RGD 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cytoskeleton  IDA 1625052 RGD 
cytosol  IDA 1625052 RGD 
membrane  IDA 1625052 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cytoskeletal protein binding enablesIDA 1625052PMID:17150188BHF-UCL 

Objects Annotated

Genes (Rattus norvegicus)
Capn10  (calpain 10)


Additional Information