RGD Reference Report - LncRNA H19 is involved in myocardial ischemic preconditioning via increasing the stability of nucleolin protein. - Rat Genome Database

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LncRNA H19 is involved in myocardial ischemic preconditioning via increasing the stability of nucleolin protein.

Authors: Chen, Cheng  Liu, Meidong  Tang, Yuting  Sun, Hui  Lin, Xiaofang  Liang, Pengfei  Jiang, Bimei 
Citation: Chen C, etal., J Cell Physiol. 2020 Sep;235(9):5985-5994. doi: 10.1002/jcp.29524. Epub 2020 Jan 24.
RGD ID: 156430334
Pubmed: PMID:31975412   (View Abstract at PubMed)
DOI: DOI:10.1002/jcp.29524   (Journal Full-text)

Myocardial ischemic preconditioning (IP) is defined as a brief period of myocardial ischemia/reperfusion (I/R) that significantly reduces injury during the subsequent exposure to long-term I/R. However, the underlying mechanisms of myocardial IP are yet to be elucidated. This study investigated the expression and roles of long noncoding RNA (lncRNA) H19 in myocardial IP in vitro and in vivo. LncRNA H19 expression levels were analyzed by quantitative reverse-transcription polymerase chain reaction, cell viability was determined by the Cell Counting Kit-8 assay, apoptosis was evaluated based on the caspase 3 activity, and RNA immunoprecipitation was performed to examine the interaction between lncRNA H19 and nucleolin. The results of this study showed that lncRNA H19 expression was significantly upregulated in mouse hearts subjected to myocardial IP, in rat H9C2 cells exposed to H2 O2 preconditioning (H2 O2 -PC), and in neonatal rat cardiomyocytes subjected to hypoxia preconditioning. H19 knockdown abrogated the H2 O2 -PC-mediated protection in cardiomyocytes evidenced by the decreased cell viability and increased caspase-3 activity. Conversely, H19 overexpression enhanced the protective role of H2 O2 -PC in cardiomyocytes. In addition, H19 overexpression increased the expression of nucleolin, whereas H19 ablation abrogated H2 O2 -PC-induced upregulation of nucleolin in cardiomyocytes. Furthermore, H19 overexpression increased the stabilization of nucleolin; an interaction between H19 and nucleolin was identified using the RNA-protein interaction studies. Furthermore, nucleolin small interfering RNA relieved the protective role of lncRNA H19. These findings demonstrated that the lncRNA H19 is involved in myocardial IP via increasing the stability of nucleolin protein and lncRNA H19 may represent a potential therapeutic target for the treatment of the myocardial injury.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
negative regulation of cardiac muscle cell apoptotic process  IMP 156430334 RGD 

Objects Annotated

Genes (Rattus norvegicus)
H19  (H19 imprinted maternally expressed transcript)


Additional Information