RGD Reference Report - Involvement of renal corpuscle microRNA expression on epithelial-to-mesenchymal transition in maternal low protein diet in adult programmed rats. - Rat Genome Database

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Involvement of renal corpuscle microRNA expression on epithelial-to-mesenchymal transition in maternal low protein diet in adult programmed rats.

Authors: Sene, Letícia de Barros  Mesquita, Flávia Fernandes  de Moraes, Leonardo Nazário  Santos, Daniela Carvalho  Carvalho, Robson  Gontijo, José Antônio Rocha  Boer, Patrícia Aline 
Citation: Sene Lde B, etal., PLoS One. 2013 Aug 19;8(8):e71310. doi: 10.1371/journal.pone.0071310. eCollection 2013.
RGD ID: 155882570
Pubmed: PMID:23977013   (View Abstract at PubMed)
PMCID: PMC3747155   (View Article at PubMed Central)
DOI: DOI:10.1371/journal.pone.0071310   (Journal Full-text)

Prior study shows that maternal protein-restricted (LP) 16-wk-old offspring have pronounced reduction of nephron number and arterial hypertension associated with unchanged glomerular filtration rate, besides enhanced glomerular area, which may be related to glomerular hyperfiltration/overflow and which accounts for the glomerular filtration barrier breakdown and early glomerulosclerosis. In the current study, LP rats showed heavy proteinuria associated with podocyte simplification and foot process effacement. TGF-β1 glomerular expression was significantly enhanced in LP. Isolated LP glomeruli show a reduced level of miR-200a, miR-141, miR-429 and ZEB2 mRNA and upregulated collagen 1α1/2 mRNA expression. By western blot analyzes of whole kidney tissue, we found significant reduction of both podocin and nephrin and enhanced expression of mesenchymal protein markers such as desmin, collagen type I and fibronectin. From our present knowledge, these are the first data showing renal miRNA modulation in the protein restriction model of fetal programming. The fetal-programmed adult offspring showed pronounced structural glomerular disorders with an accentuated and advanced stage of fibrosis, which led us to state that the glomerular miR-200 family would be downregulated by TGF-β1 action inducing ZEB 2 expression that may subsequently cause glomeruli epithelial-to-mesenchymal transition.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Fetal Nutrition Disorders  ISOCol1a1 (Rattus norvegicus)155882570; 155882570associated with maternal low protein diet and mRNA:increased expression:glomeruli (rat)RGD 
Fetal Nutrition Disorders  ISOCol1a2 (Rattus norvegicus)155882570; 155882570associated with maternal low protein diet and mRNA:increased expression:glomeruli (rat)RGD 
Fetal Nutrition Disorders  IEP 155882570; 155882570; 155882570associated with maternal low protein diet and mRNA:increased expression:glomeruli (rat)RGD 
Fetal Nutrition Disorders  ISONphs1 (Rattus norvegicus)155882570; 155882570associated with maternal low protein diet and protein:decreased expression:kidney (rat)RGD 
Fetal Nutrition Disorders  ISONphs2 (Rattus norvegicus)155882570; 155882570associated with maternal low protein diet and protein:decreased expression:kidney (rat)RGD 
Fetal Nutrition Disorders  IEP 155882570; 155882570associated with maternal low protein diet and protein:decreased expression:kidney (rat)RGD 
Fetal Nutrition Disorders  ISOZeb2 (Rattus norvegicus)155882570; 155882570associated with maternal low protein diet and mRNA:increased expression:glomeruli (rat)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Col1a1  (collagen type I alpha 1 chain)
Col1a2  (collagen type I alpha 2 chain)
Nphs1  (NPHS1 adhesion molecule, nephrin)
Nphs2  (NPHS2 stomatin family member, podocin)
Zeb2  (zinc finger E-box binding homeobox 2)

Genes (Mus musculus)
Col1a1  (collagen, type I, alpha 1)
Col1a2  (collagen, type I, alpha 2)
Nphs1  (nephrosis 1, nephrin)
Nphs2  (nephrosis 2, podocin)
Zeb2  (zinc finger E-box binding homeobox 2)

Genes (Homo sapiens)
COL1A1  (collagen type I alpha 1 chain)
COL1A2  (collagen type I alpha 2 chain)
NPHS1  (NPHS1 adhesion molecule, nephrin)
NPHS2  (NPHS2 stomatin family member, podocin)
ZEB2  (zinc finger E-box binding homeobox 2)


Additional Information