RGD Reference Report - Germline ablation of dermatan-4O-sulfotransferase1 reduces regeneration after mouse spinal cord injury. - Rat Genome Database

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Germline ablation of dermatan-4O-sulfotransferase1 reduces regeneration after mouse spinal cord injury.

Authors: Rost, S  Akyüz, N  Martinovic, T  Huckhagel, T  Jakovcevski, I  Schachner, M 
Citation: Rost S, etal., Neuroscience. 2016 Jan 15;312:74-85. doi: 10.1016/j.neuroscience.2015.11.013. Epub 2015 Nov 14.
RGD ID: 155663489
Pubmed: PMID:26586562   (View Abstract at PubMed)
DOI: DOI:10.1016/j.neuroscience.2015.11.013   (Journal Full-text)

Chondroitin/dermatan sulfate proteoglycans (CSPGs/DSPGs) are major components of the extracellular matrix. Their expression is generally upregulated after injuries to the adult mammalian central nervous system, which is known for its low ability to restore function after injury. Several studies support the view that CSPGs inhibit regeneration after injury, whereas the functions of DSPGs in injury paradigms are less certain. To characterize the functions of DSPGs in the presence of CSPGs, we studied young adult dermatan-4O-sulfotransferase1-deficient (Chst14(-/-)) mice, which express chondroitin sulfates (CSs), but not dermatan sulfates (DSs), to characterize the functional outcome after severe compression injury of the spinal cord. In comparison to their wild-type (Chst14(+/+)) littermates, regeneration was reduced in Chst14(-/-) mice. No differences between genotypes were seen in the size of spinal cords, numbers of microglia and astrocytes neither in intact nor injured spinal cords after injury. Monoaminergic innervation and re-innervation of the spinal cord caudal to the lesion site as well as expression levels of glial fibrillary acidic protein (GFAP) and myelin basic protein (MBP) were similar in both genotypes, independent of whether they were injured and examined 6weeks after injury or not injured. These results suggest that, in contrast to CSPGs, DSPGs, being the products of Chst14 enzymatic activity, promote regeneration after injury of the adult mouse central nervous system.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Spinal Cord Compression exacerbatesISOChst14 (Mus musculus)155663489; 155663489 RGD 
Spinal Cord Compression exacerbatesIMP 155663489 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Chst14  (carbohydrate sulfotransferase 14)

Genes (Mus musculus)
Chst14  (carbohydrate sulfotransferase 14)

Genes (Homo sapiens)
CHST14  (carbohydrate sulfotransferase 14)


Additional Information